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Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development
- Source :
- Journal of Immunology Research, Vol 2021 (2021), Journal of Immunology Research
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Circular RNA (circRNA) is a type of noncoding RNA that can interact with miRNAs to regulate gene expression. However, little is known concerning circRNA, which is crucial in the pathogenesis of lung cancer. To date, limited studies have explored the role of circ_0044516 in lung cancer progression. Recently, we observed that circ_0044516 expression levels were obviously elevated in lung cancer tissues and cells. A549 and SPCA1 cells were transfected with circ_0044516 siRNA. We observed that knockdown of circ_0044516 dramatically repressed cell proliferation, increased cell apoptosis, and repressed the cell cycle. Moreover, A549 and SPCA1 cell migration and invasion abilities were greatly repressed by circ_0044516 siRNA. Due to accumulating evidence demonstrating the vital role of cancer stem cells, their mechanism of involvement has drawn increasing attention in tumor progression and metastasis research. We also found that cancer stem cell properties were restrained by silencing circ_0044516 in A549 and SPC-A1 cells. Moreover, in vivo xenograft experiments showed that circ_0044516 downregulation reduced tumor growth. Mechanistically, in lung cancer and using bioinformatics, we demonstrated that circ_0044516 sponges miR-136 targeting MAT2A. Furthermore, rescue assays were carried out to identify that circ_0044516 modulates cell proliferation, invasion, and stemness by regulating miR-136 and MAT2A in lung cancer. In summary, our study revealed that the circ_0044516/miR-136/MAT2A axis is involved in lung cancer progression. Our findings may provide novel targets for diagnosis and therapeutic intervention in lung cancer patients.
- Subjects :
- 0301 basic medicine
Lung Neoplasms
Article Subject
Immunology
Biology
Metastasis
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cancer stem cell
microRNA
medicine
Animals
Humans
Immunology and Allergy
Gene silencing
Neoplasm Invasiveness
Lung cancer
Cell growth
Methionine Adenosyltransferase
RNA, Circular
General Medicine
RC581-607
Cell cycle
medicine.disease
Xenograft Model Antitumor Assays
Rats
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
A549 Cells
Tumor progression
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Disease Progression
Cancer research
Female
Immunologic diseases. Allergy
Research Article
Subjects
Details
- ISSN :
- 23147156 and 23148861
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- Journal of Immunology Research
- Accession number :
- edsair.doi.dedup.....7348499739987ab089fd3654ea05e832