Back to Search
Start Over
A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
- Source :
- PeerJ, PeerJ, Vol 4, p e1600 (2016)
- Publication Year :
- 2016
- Publisher :
- PeerJ Inc., 2016.
-
Abstract
- Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease, which sometimes makes histopathologic analysis of lesions difficult as it may not be possible to predict where lesions will occur, especially when evaluating cross sections of spinal cord. Consequently, lesions may be easily missed due to limited sampling in traditional approaches. To evaluate the entire length of the spinal cord while maintaining anatomic integrity, we have developed a method to section the cord within the decalcified spinal column, which allows for the study of the multifocal nature of this disease and also minimizes handling artifact. HE and Luxol fast blue staining of these spinal cord sections revealed a paucity of lesions in some areas, while others showed marked inflammation and demyelination. The percentage of spinal cord affected by EAE was evaluated at four separate areas of longitudinally sectioned cord and it varied greatly within each animal. Immunohistochemical staining of in situ spinal cords which had undergone decalcification was successful for key immuno-markers used in EAE research including CD3 for T cells, B220 for B cells and F4/80 for murine macrophages. This method will allow investigators to look at the entire spinal cord on a single slide and evaluate the spinal cord with and without classic EAE lesions.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Cord
Histology
Central nervous system
lcsh:Medicine
Histopathology
General Biochemistry, Genetics and Molecular Biology
Luxol fast blue stain
03 medical and health sciences
Mice
0302 clinical medicine
Paralysis
medicine
Spinal cord
business.industry
EAE
General Neuroscience
Multiple sclerosis
Experimental autoimmune encephalomyelitis
lcsh:R
General Medicine
medicine.disease
Spinal column
030104 developmental biology
medicine.anatomical_structure
Neurology
medicine.symptom
General Agricultural and Biological Sciences
business
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 21678359
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....73462c9e352817ecb8bcb41f8fbf1414