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Involvement of the endocannabinoid system in the inhibition of Sindbis virus replication: a preliminary study
- Source :
- Journal of Cannabis Research, Journal of Cannabis Research, Vol 3, Iss 1, Pp 1-8 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Background Sindbis virus (Alphaviridae) is a plus-strand RNA virus that is dependent on the host cell for replication. Cannabinoid (CB) receptors are found on most human cells, including virally infected cells. Activation of cannabinoid receptors has been shown to alter normal cellular physiology. This study aimed to assess how agonist (ACEA) or antagonists/inverse agonist (AM251) of the cannabinoid receptors would alter the cellular environment and impact Sindbis virus replication. Methods Human hepatoma (Huh7) cells were used as our model for viral replication. Cells were infected with Sindbis virus (SINV) and then treated with CB agonist (ACEA) (10 μM) or antagonist/inverse agonist (AM-251) (10 μM) and virus replication was monitored. A double subgenomic Sindbis virus containing a green fluorescent protein (GFP) reporter gene inserted into a 3′ subgenomic promoter was utilized for these assays to quickly measure viral replication. GFP fluorescent cells were analyzed using flow cytometry to measure the percentage of cells expressing the viral reporter and also quantify the levels of GFP fluorescence. Result Treatment of SINV-infected Huh7 cells with CB1 receptor antagonist/inverse agonist (AM251, 10 μM) resulted in a significant decrease in viral replication, while infected cells treated with a CB1 receptor agonist (ACEA, 10 μM) resulted in a significant increase of viral infection. The data indicates that activation of CB1 receptor by cannabinoids significantly influences the ability of Sindbis virus to replicate in the host cell. Conclusion Blocking CB1 receptor activity with 10 μM AM251 reduced viral replication, but activating the CB1 receptor with 10 μM ACEA resulted in an increase in viral infection. These results indicate cannabinoids may significantly impact a virus replicating in human liver cells. Future confirmation with other viruses and cell lines will be performed to better understand the impact of cannabinoids on viral infections.
- Subjects :
- 0301 basic medicine
AM251
Agonist
Sindbis virus
medicine.drug_class
viruses
Alphavirus
Virus
SB1-1110
03 medical and health sciences
Pharmacy and materia medica
0302 clinical medicine
medicine
Inverse agonist
Receptor
Original Research
biology
Cannabinoids
Chemistry
Plant culture
Arachidonyl-2-chloroethanolamide (ACEA)
biology.organism_classification
CB1
Molecular biology
RS1-441
030104 developmental biology
Viral replication
030220 oncology & carcinogenesis
medicine.drug
Subjects
Details
- ISSN :
- 25225782
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Cannabis Research
- Accession number :
- edsair.doi.dedup.....72f703fa0e86f96510160f68feab72b9