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Trapping and structural characterisation of a covalent intermediate in vitamin B 6 biosynthesis catalysed by the Pdx1 PLP synthase
- Source :
- RSC Chemical Biology, RSC Chemical Biology, 2022, 3 (2), pp.227-230. ⟨10.1039/D1CB00160D⟩, RSC Chemical Biology, Royal Society of Chemistry, In press, ⟨10.1039/D1CB00160D⟩, 'RSC Chemical Biology ', vol: 3, pages: 227-230 (2022)
- Publication Year :
- 2022
- Publisher :
- HAL CCSD, 2022.
-
Abstract
- International audience; The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis.
- Subjects :
- Vitamin b
0303 health sciences
ATP synthase
biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
Stereochemistry
Trapping
010402 general chemistry
01 natural sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Biochemistry
0104 chemical sciences
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
chemistry
Chemistry (miscellaneous)
Covalent bond
biology.protein
PDX1
Molecular Biology
030304 developmental biology
Subjects
Details
- Language :
- English
- ISSN :
- 26330679
- Database :
- OpenAIRE
- Journal :
- RSC Chemical Biology, RSC Chemical Biology, 2022, 3 (2), pp.227-230. ⟨10.1039/D1CB00160D⟩, RSC Chemical Biology, Royal Society of Chemistry, In press, ⟨10.1039/D1CB00160D⟩, 'RSC Chemical Biology ', vol: 3, pages: 227-230 (2022)
- Accession number :
- edsair.doi.dedup.....72e4f14ccd197150f51084d9747a1f9e
- Full Text :
- https://doi.org/10.1039/D1CB00160D⟩