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Response of AMP-activated protein kinase and lactate metabolism of largemouth bass (Micropterus salmoides) under acute hypoxic stress
- Source :
- Science of The Total Environment. 666:1071-1079
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- To study adaptation of largemouth bass (Micropterus salmoides) to hypoxic stress, we investigated physiological responses and lactate metabolism of the fish under acute hypoxia. The objectives of this study were to (a) observe changes in glucose, glycogen, and lactate content; (b) detect the activity of lactate dehydrogenase (LDH) in serum, brain, heart, and liver tissues; and (c) quantify the dynamic gene expression of AMP activated protein kinase alpha (AMPKα), hypoxia-inducible factor-1 alpha (HIF-1α), monocarboxylate transporter 1 (MCT1), monocarboxylate transporter 4 (MCT4), and lactate dehydrogenase-a (LDHa) following exposure to hypoxia. The fish were subjected to two hypoxia stresses (dissolved oxygen [DO] 1.20 ± 0.2 mg/L and 3.50 ± 0.3 mg/L, respectively) for 24 h. Our results showed that hypoxic stress significantly increased the decomposition of liver glycogen and significantly increased the concentration of blood glucose; however, the muscle glycogen content was not significantly decreased, which indicates that liver glycogen was the main energy source under acute hypoxia. Moreover, hypoxia led to accumulation of a large amount of lactic acid in tissues, possibly due to the activity of lactic acid dehydrogenase, but this process was delayed in the heart and brain relative to the liver. Additionally, hypoxia induced the expression of AMPKα, HIF-1α, MCT1, MCT4, and LDHa, suggesting that glycometabolism had switched from aerobic to anaerobic. Our results contribute to a better understanding of the molecular mechanisms of the response to hypoxia in largemouth bass.
- Subjects :
- Fish Proteins
medicine.medical_specialty
Environmental Engineering
010504 meteorology & atmospheric sciences
AMP-Activated Protein Kinases
010501 environmental sciences
01 natural sciences
chemistry.chemical_compound
AMP-activated protein kinase
Stress, Physiological
Internal medicine
Lactate dehydrogenase
medicine
Animals
Environmental Chemistry
Lactic Acid
Waste Management and Disposal
0105 earth and related environmental sciences
Monocarboxylate transporter
biology
Glycogen
Pollution
Oxygen
Monocarboxylate transporter 1
Endocrinology
chemistry
Monocarboxylate transporter 4
biology.protein
Bass
Energy source
Anaerobic exercise
Subjects
Details
- ISSN :
- 00489697
- Volume :
- 666
- Database :
- OpenAIRE
- Journal :
- Science of The Total Environment
- Accession number :
- edsair.doi.dedup.....72dfe769bf773150eae761fcc80ce806