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A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars
- Source :
- In vitro cellulardevelopmental biology : journal of the Tissue Culture Association. 25(10)
- Publication Year :
- 1989
-
Abstract
- Mandibular first molars from 17-d-old mouse embryos were cultured in vitro for 2 to 4 d by a simple, disposable, improved floatation method. This method consisted of using a 24-well multidish and a plastic culture chamber with a membrane filter. The improved floatation method, as well as our previous method, was capable of the three-dimensional development of tooth germs. Cytodifferentiation of odontoblasts and ameloblasts and formation of extracellular matrices were accelerated by the present culture system, in comparison with our previous method. All the molars cultivated by this method were very similar in morphology to in vivo. On Day 2 of culture the terminal cytodifferentiation of odontoblasts and the formation of predentin were ascertained in the bucco-lingual sections of the cultured molars. A thick layer of predentin was formed at the tip of the cusp and gradually decreased toward the cervical loop and the fissure between the buccal and lingual cusps. On Day 4 in vitro, secretory ameloblasts produced enamel matrix, and the mineralized enamel showed showed prismatic structure very similar to that in vivo. Dentin and predentin also were normal in ultrastructure. The extracellular matrices (enamel, dentine, and predentin) were formed in line with the pattern of the cusp and the formation of matrices normally started at the tip of the cusp. We conclude that the three-dimensional development of whole tooth germs in vitro may be very important for normal expression of the developmental program intrinsic to mouse embryonic molars.
- Subjects :
- Molar
Clinical Biochemistry
Cervical loop
Mice, Inbred Strains
Plant Science
Biology
Organ culture
Mice
Organ Culture Techniques
stomatognathic system
Dentin
medicine
Animals
Enamel paint
Cell Biology
Anatomy
stomatognathic diseases
Microscopy, Electron
medicine.anatomical_structure
Odontoblast
visual_art
visual_art.visual_art_medium
Cusp (anatomy)
Ameloblast
Biotechnology
Developmental Biology
Subjects
Details
- ISSN :
- 08838364
- Volume :
- 25
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- In vitro cellulardevelopmental biology : journal of the Tissue Culture Association
- Accession number :
- edsair.doi.dedup.....72c841de507c4fdfdf4330b565a712ac