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Neuroprotective effects of quercetin 4'-O-β-d-diglucoside on human striatal precursor cells in nutrient deprivation condition
- Source :
- Acta histochemica. 120(2)
- Publication Year :
- 2017
-
Abstract
- Several investigations have demonstrated neuroprotective effects of quercetin, a polyphenol widely present in nature, against neurotoxic chemicals, as well as in neuronal injury/neurodegenerative disease models. Most of these studies have been performed with quercetin aglycone and its metabolites, while scanty data are available on its glycosides. This study is aimed at investigating the neuroprotective effects of quercetin 3,4’-O-β- d -diglucoside (Q3,4’dG), isolated from the bulbs of the white cultivar (Allium cepa L.), using an in vitro model of human striatal precursor cells (HSPs), a primary culture isolated from the striatal primordium and previously characterized. To study the effect of Q3,4’dG on cell survival, HSPs were exposed to nutrient deprivation created by replacing culture medium with phosphate buffer saline (PBS). Our findings showed that Q3,4’dG treatment significantly promoted cell survival and strongly decreased apoptosis induced by nutrient deprivation, as evaluated by cell proliferation/death analyses. In addition, since the adhesive capacities of cells are essential for cell survival, the expression of some adhesion molecules, such as pancadherin and focal adhesion kinase, was evaluated. Interestingly, PBS exposure significantly decreased the expression of both molecules, while in the presence of Q3,4’dG this effect was prevented. This study provides evidence of a neuroprotective role exerted by Q3,4’dG and suggests its possible implication in sustaining neuronal survival for prevention and treatment of neurodegenerative disorders.
- Subjects :
- 0301 basic medicine
Histology
Cell Survival
Flavonoid
Blotting, Western
Pharmacology
Neuroprotection
Allium
Focal adhesion
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Precursor cell
Humans
Cells, Cultured
chemistry.chemical_classification
Oligodendrocyte Precursor Cells
Molecular Structure
Cell adhesion molecule
Cell growth
Cell Biology
General Medicine
Nutrients
Corpus Striatum
030104 developmental biology
Neuroprotective Agents
chemistry
Apoptosis
Quercetin
Food Deprivation
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 16180372
- Volume :
- 120
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Acta histochemica
- Accession number :
- edsair.doi.dedup.....72be5e267ae61941361e3b779224242f