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SCFPof1-ubiquitin and its target Zip1 transcription factor mediate cadmium response in fission yeast
- Source :
- The EMBO Journal. 24:599-610
- Publication Year :
- 2005
- Publisher :
- Wiley, 2005.
-
Abstract
- Ubiquitin-dependent proteolysis regulates gene expression in many eukaryotic systems. Pof1 is an essential fission yeast F-box protein that is homologous to budding yeast Met30. Temperature-sensitive pof1 mutants display acute growth arrest with small cell size. Extragenic suppressor analysis identified Zip1, a bZIP (basic leucine zipper) transcription factor, as a target for Pof1. We show Zip1 is stabilized in pof1 mutants, Pof1 binds only phosphorylated forms of Zip1, and Zip1 is ubiquitylated in vivo, indicating that Zip1 is a substrate of SCF(Pof1). Genome-wide DNA microarray assay shows that many cadmium-induced genes are under the control of Zip1, suggesting Zip1 plays a role in cadmium response. Consistently, zip1 mutants are hypersensitive to cadmium and unlike wild type, lose cell viability under this stress. Intriguingly, cadmium exposure results in upregulation of Zip1 levels and leads wild-type cells to growth arrest with reduced cell size, reminiscent of pof1 phenotypes. Our results indicate that Zip1 mediates growth arrest in cadmium response, which is essential to maintain viability. Normally growing cells prevent this response through constitutive ubiquitylation and degradation of Zip1 via SCF(Pof1).
- Subjects :
- Genes, Fungal
Mutant
Models, Biological
Article
General Biochemistry, Genetics and Molecular Biology
Suppression, Genetic
Ubiquitin
Schizosaccharomyces
Gene expression
Viability assay
Phosphorylation
Molecular Biology
Transcription factor
SKP Cullin F-Box Protein Ligases
General Immunology and Microbiology
biology
F-Box Proteins
General Neuroscience
Temperature
Wild type
biology.organism_classification
Yeast
Up-Regulation
DNA-Binding Proteins
Basic-Leucine Zipper Transcription Factors
G-Box Binding Factors
Biochemistry
Mutation
Schizosaccharomyces pombe
biology.protein
Schizosaccharomyces pombe Proteins
Carrier Proteins
Cadmium
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 14602075 and 02614189
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....72bc0269d5f461630ecb2ef4fe54fe26