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Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings
- Source :
- PLoS ONE, PLoS ONE, Vol 17, Iss 1, p e0263114 (2022)
- Publication Year :
- 2022
- Publisher :
- Public Library of Science, 2022.
-
Abstract
- In many countries a second wave of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has occurred, triggering a shortage of reagents needed for diagnosis and compromising the capacity of laboratory testing. There is an urgent need to develop methods to accelerate the diagnostic procedures. Pooling samples represents a strategy to overcome the shortage of reagents, since several samples can be tested using one reaction, significantly increasing the number and speed with which tests can be carried out. We have reported the feasibility to use a direct lysis procedure of saliva as source for RNA to SARS-CoV-2 genome detection by reverse transcription quantitative-PCR (RT-qPCR). Here, we show that the direct lysis of saliva pools, of either five or ten samples, does not compromise the detection of viral RNA. In addition, it is a sensitive, fast, and inexpensive method that can be used for massive screening, especially considering the proximity of the reincorporation of activities in universities, offices, and schools.
- Subjects :
- RNA viruses
Viral Diseases
Physiology
Coronaviruses
Biochemistry
Medical Conditions
Medicine and Health Sciences
Mass Screening
Pathology and laboratory medicine
Virus Testing
Multidisciplinary
Microbial Genetics
Gene Pool
Proteases
Medical microbiology
Body Fluids
Enzymes
Infectious Diseases
COVID-19 Nucleic Acid Testing
Viruses
Quarantine
Medicine
Viral Genetics
Anatomy
SARS CoV 2
Pathogens
Research Article
SARS coronavirus
Science
Microbiology
Sensitivity and Specificity
Extraction techniques
Diagnostic Medicine
Virology
Genetics
Humans
Saliva
Evolutionary Biology
Population Biology
SARS-CoV-2
Organisms
Viral pathogens
Biology and Life Sciences
Proteins
COVID-19
Covid 19
RNA extraction
Microbial pathogens
Research and analysis methods
Viral Genes
Enzymology
Population Genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....7299f374621826c4849a8bbab9c399f2