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Elevated F-EDN correlates with mucosal eosinophil degranulation in patients with IBS-A possible association with microbiota?
- Source :
- Casado-Bedmar, M, de-Faria, F M, Biskou, O, Lindqvist, C M, Ranasinghe, P D, Bednarska, O, Peterson, C, Walter, S A, Carlson, M & Keita, Å V 2021, ' Elevated F-EDN correlates with mucosal eosinophil degranulation in patients with IBS—A possible association with microbiota? ', Journal of Leukocyte Biology . https://doi.org/10.1002/jlb.4a0521-228r
- Publication Year :
- 2022
- Publisher :
- Uppsala universitet, Institutionen för medicinska vetenskaper, 2022.
-
Abstract
- Eosinophils have been linked to functional dyspepsia; however, less is known about their role in irritable bowel syndrome (IBS). This study tested the hypothesis of alterations in levels of fecal eosinophil-derived neurotoxin (F-EDN) and eosinophil density and degranulation within the colonic mucosa of IBS patients compared with healthy controls (HC). Colonic biopsies were collected from 37 IBS patients and 20 HC and analyzed for eosinophil numbers and local degranulation of eosinophil cationic protein (ECP) by histologic procedures. Fecal samples were collected for F-EDN and microbiota analysis. Differentiated 15HL-60 cells were used in vitro to investigate the direct effect of live bacteria on eosinophil activation measured by a colorimetric assay with o-phenylenediamine (OPD) substrate. We observed a higher number of eosinophils and increased extracellular ECP in the mucosa of IBS patients compared with HC. Moreover, F-EDN levels in IBS samples were elevated compared with HC and positively correlated to extracellular ECP. Metagenomic analysis showed significant correlations between bacterial composition and eosinophil measurements in both HC and IBS patients. In vitro experiments revealed an increased degranulation of 15HL-60 after stimulation with Salmonella typhimurium, Salmonella enterica, and Yersinia enterocolitica. To conclude, we could demonstrate alterations related to eosinophils in IBS, and, for the first time, a positive correlation between F-EDN levels and degranulated eosinophils in the colonic mucosa of IBS patients. Together our results suggest that eosinophils play a role in the pathophysiology of IBS and the mechanisms might be linked to an altered microbiota. Funding Agencies|Apotekare Hedberg Foundation; Magnus Bergvall Foundation [2018-02604]; Bengt Ihre Foundation [SLS-788111, SLS-882561]; Ruth and Richard Julin Foundation [2017-00350, 2019-00347]; County Council of Ostergotland [Lio-934618]; Mucosa Infection and Inflammation Center-MIIC; Medical Faculty, Uppsala University, Uppsala Sweden
- Subjects :
- 0301 basic medicine
HIGHLIGHTED ARTICLE
fecal eosinophil‐derived neurotoxin
Immunology
Eosinophil-Derived Neurotoxin
Gastroenterology and Hepatology
Biology
Microbiology in the medical area
Irritable Bowel Syndrome
03 medical and health sciences
0302 clinical medicine
Eosinophil activation
medicine
Extracellular
bacteria
eosinophil cationic protein
irritable bowel syndrome
fecal eosinophil-derived neurotoxin
Mikrobiologi inom det medicinska området
Gastroenterologi
Immunology and Allergy
Humans
Eosinophil degranulation
Irritable bowel syndrome
Eosinophil cationic protein
Mucous Membrane
Bacteria
Microbiota
Degranulation
Cell Biology
Eosinophil
medicine.disease
Pathophysiology
Eosinophils
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Casado-Bedmar, M, de-Faria, F M, Biskou, O, Lindqvist, C M, Ranasinghe, P D, Bednarska, O, Peterson, C, Walter, S A, Carlson, M & Keita, Å V 2021, ' Elevated F-EDN correlates with mucosal eosinophil degranulation in patients with IBS—A possible association with microbiota? ', Journal of Leukocyte Biology . https://doi.org/10.1002/jlb.4a0521-228r
- Accession number :
- edsair.doi.dedup.....727023672a8cbe50565694b29f6b1b8e