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Improvement of simultaneous determination of neutral monosaccharides and uronic acids by gas chromatography
- Source :
- Food Chemistry. 220:198-207
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Although pre-column derivatization with n-propylamine and acetic anhydride combined with the gas chromatograph was a useful method for the analysis of monosaccharide composition, failure often occurs because of lack of detail information on the mechanism as well as the operating key point in derivatization process. In this study, the key points in the derivatization (lactonization time, the amount of n-propylamine and acetic anhydride) were investigated and optimized to improve the method. Under the optimal conditions, the derivatives of seven neutral monosaccharides and two uronic acids were simultaneouly obtained, after which they were well separated and detected by GC. It was also found that all derivatives of monosaccharides were stable even stored for 20days. The linearity, sensitivity, precision, reproducibility and recovery rate of the improved method were evaluated. Thereafter, five polysaccharide samples from different sources were analyzed to validate the improved method.
- Subjects :
- Chromatography, Gas
Acetic Anhydrides
02 engineering and technology
Uronic acid
Polysaccharide
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
Limit of Detection
Organic chemistry
Monosaccharide
Derivatization
chemistry.chemical_classification
Reproducibility
Chromatography
Monosaccharides
010401 analytical chemistry
Reproducibility of Results
General Medicine
021001 nanoscience & nanotechnology
0104 chemical sciences
Monosaccharide composition
Acetic anhydride
Uronic Acids
chemistry
Gas chromatography
0210 nano-technology
Food Science
Subjects
Details
- ISSN :
- 03088146
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- Food Chemistry
- Accession number :
- edsair.doi.dedup.....7254ac241dd82d796ae7307e00070da5