Back to Search
Start Over
Platelet endothelial cell adhesion molecule-1 and vascular endothelial cadherin cooperatively regulate fibroblast growth factor-induced modulations of adherens junction functions
- Source :
- The Journal of investigative dermatology. 116(1)
- Publication Year :
- 2001
-
Abstract
- Cellular adherens junctions are formed by cadherins linked to proteins of the catenin family. In endothelial cells, not only vascular endothelial cadherin but also platelet endothelial cell adhesion molecule-1 localizes into junctions and associates with beta-catenin. To explore a putative cooperation of platelet endothelial cell adhesion molecule-1 and vascular endothelial cadherin, we analyzed transfectants expressing either platelet endothelial cell adhesion (CD31 cells) or vascular endothelial cadherin (CD144 cells) or both molecules (CD31/CD144 cells), and, for comparison, human umbilical vein endothelial cells. Basic fibroblast growth factor completely dissociated vascular endothelial cadherin/beta-catenin complexes and robustly moved beta-catenin into the nucleus in CD144 cells, whereas in CD31/CD144 cells as well as in human umbilical vein endothelial cells, fibroblast growth factor only partially dissociated the junctional complex followed by a significantly reduced nuclear translocation of beta-catenin. In contrast, in CD31 cells, the subcellular distribution of beta-catenin remained unaffected by fibroblast growth factor. As a functional consequence, fibroblast growth factor induced a complete collapse of the F-actin network in CD144 cells, a limited rearrangement of F-actin fibers in CD31/CD144 cells and no F-actin rearrangement in CD31 cells. We also analyzed the effect of fibroblast growth factor-induced rearrangement of junctions on junction permeability for leukocytes: in line with our observation that vascular endothelial cadherin was required for cells to respond to fibroblast growth factor, only in CD31/CD144 cells, but not in CD31 cells, leukocyte transmigration was significantly enhanced by fibroblast growth factor. In conclusion platelet endothelial cell adhesion molecule-1 cooperates with vascular endothelial cadherin in a mutual fashion; platelet endothelial cell adhesion molecule-1 reduces and temporarily limits fibroblast growth factor-induced dissociation of vascular endothelial cadherin/beta-catenin complexes, but requires vascular endothelial cadherin to control leukocyte transmigration in dependence of fibroblast growth factor.
- Subjects :
- Blood Platelets
Basic fibroblast growth factor
Dermatology
Biology
Vascular endothelial growth inhibitor
Biochemistry
Translocation, Genetic
chemistry.chemical_compound
Vasculogenesis
transmigration
Molecular Biology
beta Catenin
Cadherin
Fibroblast growth factor receptor 2
Cell Biology
Adherens Junctions
β-catenin
Cadherins
Cell biology
Vascular endothelial growth factor B
Fibroblast Growth Factors
Vascular endothelial growth factor A
Cytoskeletal Proteins
chemistry
Vascular endothelial growth factor C
Trans-Activators
CD31
CD144
Endothelium, Vascular
actin
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 116
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of investigative dermatology
- Accession number :
- edsair.doi.dedup.....7237cb9c180664616ca81098759544e3