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Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants
Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants
- Source :
- Genetics and Molecular Research, 13(2), 2906-2913, Genetics and Molecular Research 13 (2014) 2
- Publication Year :
- 2014
-
Abstract
- Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation. The cashew plants inoculated with the fungus expressing GFP revealed characteristic pathogen colonization by epifluorescence microscopy. Intense and bright green hyphae were observed for transformants in all extensions of mycelium cultures. The penetration of parenchyma cells near to the inoculation site, beneath the epicuticle surface, was observed prior to 25 dpi. Penetration was followed by the development of hyphae within invaded host cells. These findings provide a rapid and reproducible ATMT method for L. theobromae transformation.
- Subjects :
- Acetosyringone
Hypha
Agrobacterium
Genetic Vectors
Green Fluorescent Proteins
Hyphae
Microbiology
chemistry.chemical_compound
Transformation, Genetic
Ascomycota
Botany
Genetics
Nuts
Anacardium
Molecular Biology
Mycelium
Plant Diseases
Biointeracties and Plant Health
genetic-transformation
biology
Gummosis
Entomology & Disease Management
filamentous fungi
fungi
Gene Transfer Techniques
tool
General Medicine
Agrobacterium tumefaciens
biology.organism_classification
Plants, Genetically Modified
proteins
Transformation (genetics)
chemistry
PRI Biointeractions en Plantgezondheid
Lasiodiplodia theobromae
Subjects
Details
- ISSN :
- 16765680
- Volume :
- 13
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Genetics and molecular research : GMR
- Accession number :
- edsair.doi.dedup.....7231f2f41b573bcaf99f2b87d46ab989