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Glass-like protein condensate for the long-term storage of proteins
Glass-like protein condensate for the long-term storage of proteins
- Source :
- International Journal of Biological Macromolecules. 182:162-167
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Long-term storage of proteins at ambient temperature is required for applications in pharmaceutics and biotechnology. Lyophilization is a versatile approach for stabilizing proteins at ambient temperature, although its freezing and drying processes negatively affect the protein structure. In this study, we show a glass-like protein condensate (GLPC) as a new method for protein stabilization at ambient temperature. Various protein types, including immunoglobulin G, gamma globulin, albumin, and chymotrypsin, formed a glassy state during ultracentrifugation and natural drying, while proteins that tend to crystalize, such as hen egg-white lysozyme, did not. The GLPCs were characterized by a transparent solid state, similar to a dry glass ball. Importantly, the GLPCs were dissolved easily in saline solution at a physiological concentration, thereby retaining their native structures and functions. The GLPCs preserved their native structures even after 1 year of incubation at ambient temperature. These results provide a framework for the development of protein preservation methods at ambient temperature other than lyophilization.
- Subjects :
- Protein Denaturation
Hot Temperature
Drug Storage
02 engineering and technology
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Protein structure
Structural Biology
Molecular Biology
Incubation
030304 developmental biology
0303 health sciences
Chymotrypsin
biology
Protein Stability
Chemistry
Albumin
Blood Proteins
General Medicine
021001 nanoscience & nanotechnology
Vitrification
Chemical engineering
biology.protein
Pharmaceutics
Ultracentrifuge
Lysozyme
Protein stabilization
0210 nano-technology
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 182
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....721f79b906ab0fb35395e46ce90882e2
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2021.04.017