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Glass-like protein condensate for the long-term storage of proteins

Glass-like protein condensate for the long-term storage of proteins

Authors :
Suguru Nishinami
Kentaro Shiraki
Yoshitaka Nakauchi
Source :
International Journal of Biological Macromolecules. 182:162-167
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Long-term storage of proteins at ambient temperature is required for applications in pharmaceutics and biotechnology. Lyophilization is a versatile approach for stabilizing proteins at ambient temperature, although its freezing and drying processes negatively affect the protein structure. In this study, we show a glass-like protein condensate (GLPC) as a new method for protein stabilization at ambient temperature. Various protein types, including immunoglobulin G, gamma globulin, albumin, and chymotrypsin, formed a glassy state during ultracentrifugation and natural drying, while proteins that tend to crystalize, such as hen egg-white lysozyme, did not. The GLPCs were characterized by a transparent solid state, similar to a dry glass ball. Importantly, the GLPCs were dissolved easily in saline solution at a physiological concentration, thereby retaining their native structures and functions. The GLPCs preserved their native structures even after 1 year of incubation at ambient temperature. These results provide a framework for the development of protein preservation methods at ambient temperature other than lyophilization.

Details

ISSN :
01418130
Volume :
182
Database :
OpenAIRE
Journal :
International Journal of Biological Macromolecules
Accession number :
edsair.doi.dedup.....721f79b906ab0fb35395e46ce90882e2
Full Text :
https://doi.org/10.1016/j.ijbiomac.2021.04.017