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Scintigraphic Imaging of Matrix Metalloproteinase Activity in the Arterial Wall In Vivo

Authors :
Bodo Levkau
Marilyn P. Law
Michael Schäfers
Stefan Wagner
Klaus P. Schäfers
Hans-Jörg Breyholz
Burkhard Riemann
Otmar Schober
Klaus Kopka
Source :
Circulation. 109:2554-2559
Publication Year :
2004
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2004.

Abstract

Background— Matrix metalloproteinases (MMPs) are enzymes involved in the proteolytic degradation of extracellular matrix. They play an important role in several disease processes, such as inflammation, cancer, and atherosclerosis. Methods and Results— In this study, we have used the broad-spectrum MMP inhibitor CGS 27023A to develop the radioligand [ 123 I]I-HO-CGS 27023A for in vivo imaging of MMP activity. Using this radioligand, we were able to specifically image MMP activity by scintigraphy in vivo in the MMP-rich vascular lesions that develop after carotid artery ligation and cholesterol-rich diet in apolipoprotein E-deficient mice. These results were confirmed by gamma counting of lesional tissue (counts per minute per milligram). Conclusions— Imaging of MMP activity in vivo is feasible using radiolabeled MMP inhibitors. Additional studies are needed to test the potential of this approach as a novel noninvasive clinical diagnostic tool for the management of human MMP-related diseases.

Details

ISSN :
15244539 and 00097322
Volume :
109
Database :
OpenAIRE
Journal :
Circulation
Accession number :
edsair.doi.dedup.....7218c018f24b537694b8eb0b9da1e002