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Scintigraphic Imaging of Matrix Metalloproteinase Activity in the Arterial Wall In Vivo
- Source :
- Circulation. 109:2554-2559
- Publication Year :
- 2004
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2004.
-
Abstract
- Background— Matrix metalloproteinases (MMPs) are enzymes involved in the proteolytic degradation of extracellular matrix. They play an important role in several disease processes, such as inflammation, cancer, and atherosclerosis. Methods and Results— In this study, we have used the broad-spectrum MMP inhibitor CGS 27023A to develop the radioligand [ 123 I]I-HO-CGS 27023A for in vivo imaging of MMP activity. Using this radioligand, we were able to specifically image MMP activity by scintigraphy in vivo in the MMP-rich vascular lesions that develop after carotid artery ligation and cholesterol-rich diet in apolipoprotein E-deficient mice. These results were confirmed by gamma counting of lesional tissue (counts per minute per milligram). Conclusions— Imaging of MMP activity in vivo is feasible using radiolabeled MMP inhibitors. Additional studies are needed to test the potential of this approach as a novel noninvasive clinical diagnostic tool for the management of human MMP-related diseases.
- Subjects :
- Pathology
medicine.medical_specialty
Carotid Artery, Common
Matrix metalloproteinase inhibitor
Matrix Metalloproteinase Inhibitors
Matrix metalloproteinase
Hydroxamic Acids
Scintigraphy
Iodine Radioisotopes
Extracellular matrix
Mice
Apolipoproteins E
In vivo
Physiology (medical)
Radioligand
Animals
Medicine
Protease Inhibitors
Radionuclide Imaging
Ligation
Mice, Knockout
Sulfonamides
medicine.diagnostic_test
business.industry
Macrophages
Mice, Inbred C57BL
Matrix Metalloproteinase 9
Pyrazines
Autoradiography
Diet, Atherogenic
Feasibility Studies
Matrix Metalloproteinase 2
Radiopharmaceuticals
Counts per minute
Cardiology and Cardiovascular Medicine
business
Preclinical imaging
Subjects
Details
- ISSN :
- 15244539 and 00097322
- Volume :
- 109
- Database :
- OpenAIRE
- Journal :
- Circulation
- Accession number :
- edsair.doi.dedup.....7218c018f24b537694b8eb0b9da1e002