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The metastatic suppressor NDRG1 inhibits EMT, migration and invasion through interaction and promotion of caveolin-1 ubiquitylation in human colorectal cancer cells
- Source :
- Oncogene
- Publication Year :
- 2016
-
Abstract
- N-myc downstream-regulated gene 1 (NDRG1) has been reported to act as a key regulatory molecule in tumor progression-related signaling pathways, especially in tumor metastasis. However, the related mechanism has not been fully discovered yet. Herein we demonstrated that the novel molecule of cell migration and invasion, caveolin-1, has direct interaction with NDRG1 in human colorectal cancer (CRC) cells. Moreover, we discovered that NDRG1 reduces caveolin-1 protein expression through promoting its ubiquitylation and subsequent degradation via the proteasome in CRC cells. In addition, caveolin-1 mediates the suppressive function of NDRG1 in epithelial–mesenchymal transition, migration and invasion in vitro and metastasis in vivo. These results help to fulfill the potential mechanisms of NDRG1 in anti-metastatic treatment for human colorectal cancer.
- Subjects :
- 0301 basic medicine
Cancer Research
Epithelial-Mesenchymal Transition
Colorectal cancer
Caveolin 1
Immunoblotting
Fluorescent Antibody Technique
Mice, Nude
Cell Cycle Proteins
Biology
medicine.disease_cause
Real-Time Polymerase Chain Reaction
Molecular oncology
Metastasis
03 medical and health sciences
Mice
0302 clinical medicine
Cell Movement
Cell Line, Tumor
Genetics
medicine
Animals
Humans
Immunoprecipitation
Neoplasm Invasiveness
Molecular Biology
Mice, Inbred BALB C
Intracellular Signaling Peptides and Proteins
Ubiquitination
Cancer
Cell migration
Cell cycle
medicine.disease
Immunohistochemistry
Gene Expression Regulation, Neoplastic
030104 developmental biology
030220 oncology & carcinogenesis
Immunology
Cancer research
Heterografts
Original Article
Carcinogenesis
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 14765594
- Volume :
- 36
- Issue :
- 30
- Database :
- OpenAIRE
- Journal :
- Oncogene
- Accession number :
- edsair.doi.dedup.....71ebc997d72a23b752d5709b96ccf6fc