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No detection of XMRV in blood samples and tissue sections from prostate cancer patients in Northern Europe
- Source :
- PLoS ONE, Vol 6, Iss 10, p e25592 (2011), PLoS ONE
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- BACKGROUND: We recently published the rare detection of xenotropic murine leukemia virus-related virus (XMRV) (1/105) in prostate cancer (PCA) tissue of patients in Northern Europe by PCR. The controversial discussion about the virus being detected in PCA tissue, blood samples from patients suffering from chronic fatigue syndrome (CFS), as well as from a significant number of healthy controls prompted us to deepen our studies about detection of XMRV infection applying different detection methods (PCR, cocultivation and immunohistochemistry [IHC]). METHODOLOGY/PRINCIPAL FINDINGS: Peripheral blood mononuclear cells (PBMCs) from 92 PCA and 7 healthy controls were isolated, PHA activated and cocultivated with LNCaP cells for up to 8 weeks. Supernatant of these cells was applied to a reporter cell line, DERSE-iGFP. Furthermore, the PBMCs and cocultivated LNCaP cells were tested for the presence of XMRV by PCR as well as Western Blot analysis. While all PCR amplifications and Western Blot analyses were negative for signs of XMRV infection, DERSE-iGFP cells displayed isolated GFP positive cells in three cases. In all three cases XMRV presence could not be confirmed by PCR technology. In addition, we performed XMRV specific IHC on PCA tissue sections. Whole tissue sections (n = 20), as well as tissue microarrays (TMA) including 50 benign prostate hyperplasia (BPH), 50 low grade and 50 high grade PCA sections and TMAs including breast cancer, colon cancer and normal tissues were stained with two XMRV specific antisera. XMRV protein expression was not detected in any cancer sections included. One BPH tissue displayed XMRV specific protein expression in random isolated basal cells. CONCLUSION: We were unable to conclusively detect XMRV in the blood from PCA patients or from healthy controls and there is no conclusive evidence of XMRV protein expression in PCA, breast cancer and colon cancer tissue sections tested by IHC staining.
- Subjects :
- Male
Pathology
Epidemiology
Xenotropic murine leukemia virus-related virus
lcsh:Medicine
Cell Separation
Lymphocyte Activation
Virus Replication
urologic and male genital diseases
Polymerase Chain Reaction
Prostate cancer
Emerging Viral Diseases
lcsh:Science
Multidisciplinary
Tissue microarray
biology
Cancer Risk Factors
Prostate Cancer
Prostate
virus diseases
Middle Aged
Immunohistochemistry
Europe
Leukemia
Oncology
Medicine
Cancer Epidemiology
Research Article
Adult
medicine.medical_specialty
Viral and Bacterial Causes of Cancer
Microbiology
Peripheral blood mononuclear cell
Viral Proteins
Cell Line, Tumor
Virology
Retroviruses
LNCaP
medicine
Humans
Viral Nucleic Acid
Biology
Aged
lcsh:R
Prostatic Neoplasms
Cancers and Neoplasms
Cancer
biology.organism_classification
medicine.disease
Coculture Techniques
Viral Replication
Genitourinary Tract Tumors
Viral Classification
Case-Control Studies
Leukocytes, Mononuclear
lcsh:Q
Retroviridae Infections
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....71c84222422854dae26a8d652629fd4f