Back to Search
Start Over
Mosaic hepatitis B virus core particles presenting the complete preS sequence of the viral envelope on their surface
- Source :
- Journal of General Virology. 85:2665-2670
- Publication Year :
- 2004
- Publisher :
- Microbiology Society, 2004.
-
Abstract
- The sequence of the preS domain of the hepatitis B virus (HBV, genotype D) envelope was inserted into the major immunodominant region (MIR) of the C-terminally truncated HBV core (HBc) protein. In Escherichia coli, the HBc–preS fusion protein was partially soluble and did not produce particles. Co-expression of the wild-type HBc as a helper protein along with the fusion protein led to the formation of mosaic HBc particles that exhibited HBc, preS1 and preS2 antigenicity. Two alternative combinations of medium- and high-copy plasmids were used for co-expression of fusion and helper proteins, in an attempt to improve mosaic particle production. However, the preS fusion content of the particles remained the same in both expression combinations. In a third co-expression in which the modified HBc helper lacked aa 76–85 in the MIR, the incorporation level of HBc–preS fusion into the particles was noticeably lower. Purified chimeric particles were immunogenic in mice.
- Subjects :
- Hepatitis B virus
Antigenicity
viruses
Antibodies, Viral
Protein Engineering
medicine.disease_cause
Virus
Mice
Viral Envelope Proteins
Orthohepadnavirus
Viral envelope
Virology
Escherichia coli
medicine
Animals
Protein Precursors
Hepatitis B Surface Antigens
biology
Viral Core Proteins
virus diseases
Protein engineering
Hepatitis B
biology.organism_classification
Fusion protein
digestive system diseases
Hepadnaviridae
Female
Immunization
Reassortant Viruses
Plasmids
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....71b9a571d9910617f18669b008e8fbec
- Full Text :
- https://doi.org/10.1099/vir.0.79810-0