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Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy

Authors :
Dan L. Sackett
Randall Pursley
Soongho Park
Claude Boccara
Amir H. Gandjbakhche
Emilie Benoit
Marcial Garmendia-Cedillos
Thien Nguyen
Source :
Biomedical optics express. 12(10)
Publication Year :
2021

Abstract

Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.

Details

ISSN :
21567085
Volume :
12
Issue :
10
Database :
OpenAIRE
Journal :
Biomedical optics express
Accession number :
edsair.doi.dedup.....71a452745caff5584b892c8e72e7769c