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Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast
- Source :
- Molecular Biology of the Cell
- Publication Year :
- 2021
- Publisher :
- American Society for Cell Biology (ASCB), 2021.
-
Abstract
- Proper mitotic progression inSchizosaccharomyces pomberequires partial nuclear envelope breakdown (NEBD) and insertion of the spindle pole body (SPB – yeast centrosome) to build the mitotic spindle. Linkage of the centromere to the SPB is vital to this process, but why that linkage is important is not well understood. Utilizing high- resolution structured illumination microscopy (SIM), we show that the conserved SUN- domain protein Sad1 and other SPB proteins redistribute during mitosis to form a ring complex around SPBs, which is a precursor for localized NEBD and spindle formation. Although the Polo kinase Plo1 is not necessary for Sad1 redistribution, it localizes to the SPB region connected to the centromere, and its activity is vital for redistribution of other SPB ring proteins and for complete NEBD at the SPB to allow for SPB insertion. Our results lead to a model in which centromere linkage to the SPB drives redistribution of Sad1 and Plo1 activation that in turn facilitate partial NEBD and spindle formation through building of a SPB ring structure.
- Subjects :
- Nuclear Envelope
Centromere
Mitosis
Spindle Apparatus
macromolecular substances
Protein Serine-Threonine Kinases
Biology
Spindle pole body
03 medical and health sciences
0302 clinical medicine
Schizosaccharomyces
Molecular Biology
030304 developmental biology
Centrosome
0303 health sciences
Chemistry
Polo kinase
Nuclear Proteins
Articles
Cell Biology
biology.organism_classification
Spindle apparatus
Cell biology
Protein Transport
Spindle Pole Bodies
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins
Multipolar spindles
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....719ba1784a3936e90fc861ddff1e4117