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The effects of group and single housing and automated animal monitoring on urinary corticosterone levels in male C57BL/6 mice
- Source :
- Physiological Reports
- Publication Year :
- 2016
- Publisher :
- John Wiley & Sons, 2016.
-
Abstract
- Mice are used extensively in physiological research. Automated home‐cage systems have been developed to study single‐housed animals. Increased stress by different housing conditions might affect greatly the results when investigating metabolic responses. Urinary corticosteroid concentration is considered as a stress marker. The aim of the study was to compare the effects of different housing conditions and an automated home‐cage system with indirect calorimetry located in an environmental chamber on corticosterone levels in mice. Male mice were housed in different conditions and in automated home‐cage system to evaluate the effects of housing and measuring conditions on urine corticosterone levels. Corticosterone levels in single‐housed mice in the laboratory animal center were consistently lower compared with the group‐housed mice. Single‐housed mice in a separate, small animal unit showed a rise in their corticosterone levels a day after they were separated to their individual cages, which decreased during the following 2 days. The corticosterone levels of group‐housed mice in the same unit were increased during the first 7 days and then decreased. On day 7, the corticosterone concentrations of group‐housed mice were significantly higher compared with that of single‐housed mice, including the metabolic measurement protocol. In conclusion, single housing caused less stress when compared with group‐housed mice. In addition, the urine corticosterone levels were decreased in single‐housed mice before the metabolic measurement started. Thus, stress does not affect the results when utilizing the automated system for measuring metabolic parameters like food and water intake and calorimetry.
- Subjects :
- 0301 basic medicine
C57BL/6
Male
medicine.medical_specialty
mice
Physiology
medicine.drug_class
Urinary system
Urine
Animal monitoring
03 medical and health sciences
chemistry.chemical_compound
Automation
stress
0302 clinical medicine
Corticosterone
Physiology (medical)
Small animal
Internal medicine
medicine
Metabolism and Regulation
Animals
Renal Filtration
Cellular and Molecular Endocrinology
Original Research
biology
biology.organism_classification
Housing, Animal
Stress marker
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
chemistry
urinary corticosterone
Environmental Physiology
metabolic measurements
Corticosteroid
030217 neurology & neurosurgery
Stress, Psychological
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Physiological Reports
- Accession number :
- edsair.doi.dedup.....71965d5033722df6d0fe1cbeca7d7284