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Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
- Source :
- Brazilian Archives of Biology and Technology, Volume: 50, Issue: spe, Pages: 123-128, Published: SEP 2007, Brazilian Archives of Biology and Technology, Vol 50, Iss spe, Pp 123-128 (2007), Brazilian Archives of Biology and Technology v.50 n.spe 2007, Brazilian Archives of Biology and Technology, Instituto de Tecnologia do Paraná (Tecpar), instacron:TECPAR
- Publication Year :
- 2007
- Publisher :
- FapUNIFESP (SciELO), 2007.
-
Abstract
- The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (pO objetivo desse trabalho foi avaliar o efeito da Artemisia vulgaris L.(artemisa) na marcação dos constituintes sangüíneos com tecnécio-99m (99mTc). Amostras de sangue obtidas de ratos Wistar foram incubadas com um extrato de artemisa e o processo de radiomarcação dos constituintes sangüíneos foi realizado. Plasma e células sangüíneas foram isoladas por centrifugação. Alíquotas de plasma e células sangüíneas foram também precipitadas com ácido tricloroacético para isolamento de frações solúvel e insolúvel. A radiatividade em cada fração foi contada e as porcentagens de radioatividade (%ATI) foram calculadas. O extrato de artemisa diminuiu significantemente (p
- Subjects :
- Multidisciplinary
Chromatography
biology
Chemistry
lcsh:Biotechnology
Technetium-99m
Blood constituents
Artemisia vulgaris L
biology.organism_classification
Blood proteins
In vitro
chemistry.chemical_compound
Mugwort
lcsh:TP248.13-248.65
Centrifugation
Trichloroacetic acid
labeling
Artemisia vulgaris
Subjects
Details
- ISSN :
- 15168913
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Brazilian Archives of Biology and Technology
- Accession number :
- edsair.doi.dedup.....718c03f2324e14fdde38ba0d57f00342
- Full Text :
- https://doi.org/10.1590/s1516-89132007000600015