Back to Search
Start Over
Human Adipose-Derived Stromal Cells Efficiently Support Hematopoiesis In Vitro and In Vivo: A Key Step for Therapeutic Studies
- Source :
- Stem Cells and Development. 20:2127-2138
- Publication Year :
- 2011
- Publisher :
- Mary Ann Liebert Inc, 2011.
-
Abstract
- Adipose-derived stromal cells (ADSCs) are close relatives of bone marrow mesenchymal stromal cells (BM-MSCs). The ease of access to subcutaneous fat pad and the abundance of stromal precursors make fat tissue an attractive source of stromal cells for clinicians. However, their ability to support hematopoietic stem cells in vitro and in vivo has not been established definitively. Thus, their usefulness in supporting hematopoietic stem cell engraftment is not as clear as with BM-MSCs. In this article, we show that human ADSCs, cultured with a good manufacturing practice medium, maintain in vitro human early and committed hematopoietic progenitors and support their complete differentiation toward myeloid and lymphoid lineages. Compared with BM-MSCs, ADSCs elicit a more precocious early progenitor formation and faster proliferation and differentiation of hematopoietic progenitors. Further, in vivo, when co-injected in NOD.Cg-Prkdc(scid) Il2(rgtm1Wjl)/SzJ (NSG) mice with a low number of human CD34(+) cells, ADSCs enabled the higher production of immature human hematopoietic progenitors and CD45(+) cells when compared with BM-MSCs. As a whole, our results indicate that human ADSCs, isolated and expanded under clinical-grade conditions, support hematopoiesis in vitro and in vivo and thus provide the rationale for their use in supporting hematopoietic reconstitution in clinical settings.
- Subjects :
- Adult
Male
Stromal cell
Adipose tissue
Antigens, CD34
Biology
Mesenchymal Stem Cell Transplantation
Subcutaneous fat
Mice
In vivo
medicine
Animals
Humans
Cell Lineage
Aged
Cell Proliferation
Aged, 80 and over
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Hematology
Middle Aged
Hematopoietic Stem Cells
In vitro
Hematopoiesis
Haematopoiesis
medicine.anatomical_structure
Adipose Tissue
Immunology
Cancer research
Female
Bone marrow
Developmental Biology
Subjects
Details
- ISSN :
- 15578534 and 15473287
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Stem Cells and Development
- Accession number :
- edsair.doi.dedup.....71839c238bfe55108fe067f78c0f80cd