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Cell Density Dependence of Transformation Frequencies in C3H10T1/2 Cells Exposed to X-rays
- Source :
- International Journal of Radiation Biology. 56:989-998
- Publication Year :
- 1989
- Publisher :
- Informa UK Limited, 1989.
-
Abstract
- The effects of cell density on transformation frequencies were studied in C3H10T1/2 cells exposed to 0.5 and 7 Gy of 200 kVp X-rays. Initial cell density strongly influenced transformation frequency; this decreased by a factor of between 4 and 10 when the initial seeding density was changed from 50 to 2500 cells/10 cm diameter Petri dish. The data were fitted with two equations: (a) an allometric function represented on a log-log scale by a straight line and (b) a sigmoidal function with plateaux between 50 and 250 cells/dish and above 600. The two curves are compared and their probabilities discussed. Our data indicate that the region between 50 and 250 cells/dish would be the most suitable region for dose-effect measurements. A study of the growth curves at 0.5 and 8.5 Gy shows that cell growth rates are not influenced by initial cell density.
- Subjects :
- Materials science
Radiological and Ultrasound Technology
Cell growth
Petri dish
Mineralogy
Cell Count
In Vitro Techniques
Molecular physics
Cell Line
law.invention
Mice
Transformation (genetics)
Cell Transformation, Neoplastic
law
Cell culture
Cell density
Animals
Initial cell
Radiology, Nuclear Medicine and imaging
Seeding
X ray irradiation
Subjects
Details
- ISSN :
- 13623095 and 09553002
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- International Journal of Radiation Biology
- Accession number :
- edsair.doi.dedup.....717abebd2e296ae8e5a4aebcecdfa8db