Additional file 1 of barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection

Additional file 1: Tables S1. Sequences for the 21 barcodes designed with the barCoder algorithm. Table S2. Primer and probe sequences for each barcode module. Table S3. Details the constraint properties of the primers and probes that were experimentally validated in this study. Table S4. Details the check results for properties of the primers and probes that were experimentally validated in this study. Table S5. Details the BLAST check results for the primers and probes that were experimentally validated in this study. Table S6. Details the spacer and barcode module checks for the barcodes experimentally validated in this study. Table S7. Sources of DNA used in the cross-reactivity panel shown in Table 4. Table S8. Sequences of the primers used to construct the barcoded strain of Y. pestis CO92 pgm−. Figure S1. Additional qPCR standard curves.