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Dispersal of single and mixed non- albicans Candida species biofilms by β-1,3-glucanase in vitro
- Source :
- Microbial Pathogenesis. 113:342-347
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- β-1,3-glucan plays a role in non-albicans Candida species biofilm formation and survival of biofilm Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1 → 3)-glucose of non-albicans Candida species biofilms, on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis and Candida krusei. Biofilm by all tested species in microplate were dispersed more than 60%. β-1,3-glucanase also detached mixed species biofilm in microplate and on medical material surface. β-1,3-glucanase had no effect on Candida planktonic growth as well as adhesion. However, further biofilm formation was inhibited with β-1,3-glucanase added at 24 h after biofilm initiation. β-1,3-glucanase markedly enhanced the antifungal susceptibility of amphotericin B. The examination using confocal laser scanning microscopy and scanning electron microscope confirmed the antibiofilm activity of β-1,3-glucanase. Our findings demonstrate that β-1,3-glucanase may be useful as an antibiofilm agent.
- Subjects :
- 0301 basic medicine
Antifungal Agents
Candida parapsilosis
beta-Glucans
030106 microbiology
Cell Communication
Microbial Sensitivity Tests
Microbiology
Candida tropicalis
03 medical and health sciences
Amphotericin B
Candida krusei
Candida
Microscopy, Confocal
biology
Biofilm
Glucan 1,3-beta-Glucosidase
Adhesion
biochemical phenomena, metabolism, and nutrition
Glucanase
biology.organism_classification
Coculture Techniques
In vitro
Drug Combinations
030104 developmental biology
Infectious Diseases
Biofilms
Microscopy, Electron, Scanning
Biological dispersal
Subjects
Details
- ISSN :
- 08824010
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Microbial Pathogenesis
- Accession number :
- edsair.doi.dedup.....7163d1609e0d237b6560f2fb4ac2af95