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Cytotoxicity evaluation of biodegradable Zn–3Mg alloy toward normal human osteoblast cells
- Source :
- Materials Science and Engineering: C. 49:560-566
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- The recent proposal of using Zn-based alloys for biodegradable implants was not supported with sufficient toxicity data. This work, for the first time, presents a thorough cytotoxicity evaluation of Zn-3Mg alloy for biodegradable bone implants. Normal human osteoblast cells were exposed to the alloy's extract and three main cell-material interaction parameters: cell health, functionality and inflammatory response, were evaluated. Results showed that at the concentration of 0.75mg/ml alloy extract, cell viability was reduced by ~50% through an induction of apoptosis at day 1; however, cells were able to recover at days 3 and 7. Cytoskeletal changes were observed but without any significant DNA damage. The downregulation of alkaline phosphatase protein levels did not significantly affect the mineralization process of the cells. Significant differences of cyclooxygenase-2 and prostaglandin E2 inflammatory biomarkers were noticed, but not interleukin 1-beta, indicating that the cells underwent a healing process after exposure to the alloy. Detailed analysis on the cell-material interaction is further discussed in this paper.
- Subjects :
- Materials science
Cell Survival
Cell
Down-Regulation
Apoptosis
Biocompatible Materials
Bioengineering
Bone and Bones
Dinoprostone
Cell Line
Biomaterials
Absorbable Implants
Materials Testing
Alloys
medicine
Humans
Magnesium
Viability assay
Prostaglandin E2
Cytotoxicity
Cytoskeleton
Inflammation
Osteoblasts
Metallurgy
Interleukin
Osteoblast
Alkaline Phosphatase
Cell biology
Zinc
medicine.anatomical_structure
Cyclooxygenase 2
Mechanics of Materials
Alkaline phosphatase
Biomarkers
DNA Damage
medicine.drug
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....715c38f4b64765776676eca31c6584a4