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Whole brain in vivo neuropathology: Imaging site-specific changes in brain structure over time following trimethyltin exposure in rats
- Source :
- Toxicology letters. 352
- Publication Year :
- 2021
-
Abstract
- Presented is a diffusion weighted imaging protocol with measures of apparent diffusion coefficient which when registered to a 3D MRI rat brain atlas provides site-specific information on 173 different brain areas. This protocol coined “in vivo neuropathology” was used to follow the progressive neurotoxic effects of trimethyltin on global gray matter microarchitecture. Four rats were given an IP injection of 7 mg/kg of the neurotoxin trimethyltin and imaged for changes in water diffusivity at 3- and 7-days post injections. At 3 days, there was a significant decrease in apparent diffusion coefficient, a proxy for cytotoxic edema, in several cortical areas and cerebellum. At 7 days the level of injury expanded to include most of the cerebral cortex, hippocampus, olfactory system, and cerebellum/brainstem corroborating much of the work done with traditional histopathology. Analysis is achieved with a minimum number of rats adhering to the laws and regulations around the humane care and use of laboratory animals, providing an alternative to the traditional tests for assessing drug neurotoxicity. “In vivo neuropathology” can minimize the cost, expedite the process, and identify subtle changes in site-specific brain microarchitecture across the entire brain.
- Subjects :
- Inflammation
Male
Trimethyltin Compounds
business.industry
Neurotoxicity
Hippocampus
Brain Edema
General Medicine
Neuropathology
Toxicology
medicine.disease
Rats
Rats, Sprague-Dawley
medicine.anatomical_structure
Cerebral cortex
In vivo
medicine
Effective diffusion coefficient
Animals
Brainstem
business
Neuroscience
Diffusion MRI
Subjects
Details
- ISSN :
- 18793169
- Volume :
- 352
- Database :
- OpenAIRE
- Journal :
- Toxicology letters
- Accession number :
- edsair.doi.dedup.....7118a6efd3e70f4d894f7be97a11d339