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Diagnostic accuracy and feasibility of serological tests on filter paper samples for outbreak detection of T.b. gambiense human African trypanosomiasis
- Source :
- The American journal of tropical medicine and hygiene. 83(2)
- Publication Year :
- 2010
-
Abstract
- Control of human African trypanosomiasis (HAT) in the Democratic Republic of Congo is based on mass population screening by mobile teams; a costly and labor-intensive approach. We hypothesized that blood samples collected on filter paper by village health workers and processed in a central laboratory might be a cost-effective alternative. We estimated sensitivity and specificity of micro-card agglutination test for trypanosomiasis (micro-CATT) and enzyme-linked immunosorbent assay (ELISA)/T.b. gambiense on filter paper samples compared with parasitology-based case classification and used the results in a Monte Carlo simulation of a lot quality assurance sampling (LQAS) approach. Micro-CATT and ELISA/T.b. gambiense showed acceptable sensitivity (92.7% [95% CI 87.4-98.0%] and 82.2% [95% CI 75.3-90.4%]) and very high specificity (99.4% [95% CI 99.0-99.9%] and 99.8% [95% CI 99.5-100%]), respectively. Conditional on high sample size per lot (> or = 60%), both tests could reliably distinguish a 2% from a zero prevalence at village level. Alternatively, these tests could be used to identify individual HAT suspects for subsequent confirmation.
- Subjects :
- Veterinary medicine
Trypanosoma brucei gambiense
Prevalence
Serological
Antibodies, Protozoan
Serology
Disease Outbreaks
Sensitivity
Direct agglutination test
Medicine
African trypanosomiasis
Evaluation
Diagnostics
Accuracy
Blood Specimen Collection
Tsetse flies
Feasibility
Articles
Protozoal diseases
Vectors
Latex fixation test
Test kits
Detection
Congo-Kinshasa
Infectious Diseases
Specificity
Democratic Republic of the Congo
ELISA
Lot quality assurance sampling
Monte Carlo Method
Paper
Enzyme-Linked Immunosorbent Assay
Filter paper
Sensitivity and Specificity
Specimen Handling
Virology
Agglutination Tests
CATT
Humans
Africa, Central
business.industry
Rapid diagnostic tests
Outbreaks
Sleeping sickness
medicine.disease
Trypanosomiasis, African
ROC Curve
Sample size determination
Parasitology
business
Trypanosomiasis
Filtration
Latex Fixation Tests
Subjects
Details
- ISSN :
- 14761645
- Volume :
- 83
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The American journal of tropical medicine and hygiene
- Accession number :
- edsair.doi.dedup.....7110b5a4cf22a26b9c46cf5d60662be7