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Purification and identification of buckwheat hull flavonoids and its comparative evaluation on antioxidant and cytoprotective activity in vitro
- Source :
- Food Science & Nutrition, Food Science & Nutrition, 8(7):3882-3892, Food Science & Nutrition, Vol 8, Iss 7, Pp 3882-3892 (2020)
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Buckwheat hulls, by‐products of buckwheat processing, contain various flavonoids, but the antioxidant capacity and synergy of different flavonoids are unclear. The purpose of this study was to evaluate the antioxidant activity of flavonoid monomers and high‐flavonoid component (HBHF) in buckwheat hulls using chemical‐based assays and cellular‐based assays. Four kinds of flavonoids were identified as rutin, isoorientin, vitexin, and hyperoside from buckwheat hulls. In chemical‐based assays, rutin and HBHF showed relatively high free radical scavenging activities and total antioxidant capacities. In cellular‐based assays, however, HBHF showed much higher proliferation activity against cell damage than flavonoid monomers when HepG2 cell was oxidatively damaged by H2O2 or high glucose. The cytoprotective activities of flavonoid monomers and HBHF were closely associated with reducing levels of reactive oxygen species (ROS) and malondialdehyde (MDA) or increasing catalase (CAT) activity. In conclusion, buckwheat hull flavonoids are the favorable application candidates for natural antioxidants.<br />This work was aimed at the evaluation of antioxidant and cell protective activities of buckwheat hull flavonoids and providing a favorable evidence for the therapeutic utilization of natural extract.
- Subjects :
- Antioxidant
cytoprotective activity
buckwheat hulls
antioxidant activity
flavonoid components
purification and identification
Isoorientin
medicine.medical_treatment
Flavonoid
Vitexin
Hyperoside
03 medical and health sciences
Rutin
chemistry.chemical_compound
0404 agricultural biotechnology
medicine
TX341-641
heterocyclic compounds
Food science
Original Research
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
biology
Nutrition. Foods and food supply
Chemistry
fungi
food and beverages
04 agricultural and veterinary sciences
040401 food science
carbohydrates (lipids)
Catalase
biology.protein
Food Science
Subjects
Details
- ISSN :
- 20487177
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Food Science & Nutrition
- Accession number :
- edsair.doi.dedup.....710dadf59eef115dea58e13021bfc53e