Supplementary Figure 3 from Comparative microRNA Profiling of Prostate Carcinomas with Increasing Tumor Stage by Deep Sequencing

PDF file - 123K, Analysis of miR-15a and miR-200b expression by Northern blotting (A) Ectopic expression of miR-15a and miR-200b. 293T cells were transfected either with pSG5 vector or with the pSG5-miR-15a or-200b expression plasmids. The ectopic expression of the miRNAs was detected by Northern blotting using specific radioactive labeled probes. (B) Endogenous expression of miR-15a and miR-200b in PCa cell lines DU145 and LNCaP. Total RNA was extracted from the cell lines PNF, DU145 and LNCaP and analyzed by Northern blotting using specific radioactive labeled probes against miR-15a and -200b. tRNA served as loading control.