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Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis
- Source :
- Journal of Spectroscopy, Vol 2014 (2014)
- Publication Year :
- 2014
- Publisher :
- Hindawi Limited, 2014.
-
Abstract
- Interaction of HMGB1 nonhistone chromosomal protein with DNA was studied using circular dichroism spectroscopy and thermal denaturation of DNA. Melting DNA in the complex was shown to be a biphasic process. The characteristic melting temperatures of unbound DNA and the DNA bound to HMGB1 in 0.25 mM EDTA solutions were found to beTmI=44.0±0.5°C andTmII=62.0±1°C, respectively. It was shown that the binding of the HMGB1 molecule affects the melting of the DNA region approximately 30 b.p. long.
- Subjects :
- Thermal denaturation
Circular dichroism
Article Subject
biology
Chemistry
chemical and pharmacologic phenomena
HMGB1
Atomic and Molecular Physics, and Optics
Analytical Chemistry
Nucleic acid thermodynamics
chemistry.chemical_compound
Biochemistry
biology.protein
Biophysics
lcsh:QC350-467
Molecule
lcsh:Optics. Light
Spectroscopy
DNA
Subjects
Details
- ISSN :
- 23144939 and 23144920
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- Journal of Spectroscopy
- Accession number :
- edsair.doi.dedup.....70fed10b6ce7a0295bf0a33fa2125f5b
- Full Text :
- https://doi.org/10.1155/2014/387138