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Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis

Authors :
E. V. Chikhirzhina
Starkova J. Tatiana
A. M. Polyanichko
Source :
Journal of Spectroscopy, Vol 2014 (2014)
Publication Year :
2014
Publisher :
Hindawi Limited, 2014.

Abstract

Interaction of HMGB1 nonhistone chromosomal protein with DNA was studied using circular dichroism spectroscopy and thermal denaturation of DNA. Melting DNA in the complex was shown to be a biphasic process. The characteristic melting temperatures of unbound DNA and the DNA bound to HMGB1 in 0.25 mM EDTA solutions were found to beTmI=44.0±0.5°C andTmII=62.0±1°C, respectively. It was shown that the binding of the HMGB1 molecule affects the melting of the DNA region approximately 30 b.p. long.

Details

ISSN :
23144939 and 23144920
Volume :
2014
Database :
OpenAIRE
Journal :
Journal of Spectroscopy
Accession number :
edsair.doi.dedup.....70fed10b6ce7a0295bf0a33fa2125f5b
Full Text :
https://doi.org/10.1155/2014/387138