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Modular Inducible Multigene Expression System for Filamentous Fungi

Modular Inducible Multigene Expression System for Filamentous Fungi

Authors :
Clara Baldin
Alexander Kühbacher
Petra Merschak
Johannes Wagener
Fabio Gsaller
Source :
Microbiology spectrum. 10(6)
Publication Year :
2022

Abstract

Inducible promoters are indispensable elements when considering the possibility to modulate gene expression on demand. Desirable traits of conditional expression systems include their capacity for tight downregulation, high overexpression, and in some instances for fine-tuning, to achieve a desired product's stoichiometry. Although the number of inducible systems is slowly increasing, suitable promoters comprising these features are rare. To date, the concomitant use of multiple regulatable promoter platforms for controlled multigene expression has been poorly explored. This work provides pioneer work in the human pathogenic fungus Aspergillus fumigatus, wherein we investigated different inducible systems, elucidated three candidate promoters, and proved for the first time that up to three systems can be used simultaneously without interfering with each other. Proof of concept was obtained by conditionally expressing three antifungal drug targets within the ergosterol biosynthetic pathway under the control of the xylose-inducible

Details

ISSN :
21650497
Volume :
10
Issue :
6
Database :
OpenAIRE
Journal :
Microbiology spectrum
Accession number :
edsair.doi.dedup.....70ef74fbeafacd9eedfba07674839fc1