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Effect of Precultivation Conditions on Colicin Susceptibility inEscherichia coli
- Source :
- Journal of Chemotherapy. 7:100-105
- Publication Year :
- 1995
- Publisher :
- Informa UK Limited, 1995.
-
Abstract
- The effect of 20 colicins and cloacin was studied after various precultivations. Nutrient agar supplemented with subinhibitory concentration of EDTA used for precultivation or elevating the growth-temperature of the inoculum from 37 degrees C to 42 degrees C increased the susceptibility of wild-type (smooth) Escherichia coli strains to the inhibitory action of some colicins. There were great differences among the colicins in respect to these effects. In case of rough mutants, their sensitivities did not change or eventually decrease after EDTA or heat pretreatment. The LPS pattern in SDS-PAGE of smooth cells grown in EDTA-containing nutrient medium changed in some degree towards the rough character. In case of precultivation at 42 degrees C this change was less considerable. It is supposed that both factors applied during precultivation have influence on colicin sensitivity by means of the change of receptor activity caused by LPS modification.
- Subjects :
- Lipopolysaccharides
Mutant
Lipopolysaccharide Receptors
Colicins
Microbial Sensitivity Tests
Biology
Cloacin
medicine.disease_cause
Heat pretreatment
Microbiology
chemistry.chemical_compound
Escherichia coli
medicine
Pharmacology (medical)
Receptors, Immunologic
Receptor
Edetic Acid
Antibacterial agent
Pharmacology
Temperature
In vitro
Culture Media
Erythromycin
Infectious Diseases
Oncology
chemistry
Colicin
Streptomycin
Ampicillin
sense organs
Nutrient agar
Subjects
Details
- ISSN :
- 19739478 and 1120009X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of Chemotherapy
- Accession number :
- edsair.doi.dedup.....70e6f88e307afc2268e8ab206d795385