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Telomere Length, Telomerase Activity, and Expressions of Human Telomerase mRNA Component (hTERC) and Human Telomerase Reverse Transcriptase (hTERT) mRNA in Pulmonary Neuroendocrine Tumors
- Source :
- Japanese Journal of Clinical Oncology. 37:16-22
- Publication Year :
- 2006
- Publisher :
- Oxford University Press (OUP), 2006.
-
Abstract
- Background: Telomeres are important for chromosome structure and function, protecting them against degradation. However, few studies have examined telomeres in pulmonary neuroendocrine (NE) tumors. Methods: We investigated deparaffinized sections obtained from 70 primary NE lung tumors [34 typical carcinoids (TCs), 10 atypical carcinoids (ACs), 16 large cell neuroendocrine carcinoma (LCNECs) and 10 small cell lung carcinomas (SCLCs)]. Results: Positive expressions of human telomerase mRNA component (hTERC) and human telomerase reverse transcriptase (hTERT) mRNA were recognized, respectively, in 58% and 74% of TCs, and in 100% and 100% of ACs, LCNECs and SCLCs. Alteration of telomere length was greater in both LCNECs and SCLCs than in TCs. Telomerase activity was detected in LCNECs, but not in TCs. By the reverse-transcriptase polymerase chain reaction (RT-PCR), hTERC mRNA was detected in 100% of LCNECs and TCs examined, while hTERT mRNA was detected in 67% of LCNECs, but not at all in TCs. Conclusions: These results suggest that alterations in telomere length, telomerase activity, and the expression of hTERT mRNA may (i) play roles in pathogenesis in pulmonary neuroendocrine tumors, and (ii) be a useful tool for differential diagnosis between TCs and LCNECs.
- Subjects :
- Cancer Research
Telomerase
Lung Neoplasms
Cell
Biology
Neuroendocrine tumors
law.invention
Pathogenesis
law
medicine
Humans
Radiology, Nuclear Medicine and imaging
Telomerase reverse transcriptase
RNA, Messenger
In Situ Hybridization
Polymerase chain reaction
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
fungi
General Medicine
Telomere
medicine.disease
Molecular biology
Carcinoma, Neuroendocrine
medicine.anatomical_structure
Oncology
RNA
Subjects
Details
- ISSN :
- 14653621 and 03682811
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Japanese Journal of Clinical Oncology
- Accession number :
- edsair.doi.dedup.....70e22595e6ace07c4eca813911a3dff3