Back to Search
Start Over
Membrane Dynamics and Organization of the Phagocyte NADPH Oxidase in PLB-985 Cells
- Source :
- Frontiers in Cell and Developmental Biology, Frontiers in Cell and Developmental Biology, 2020, 8, ⟨10.3389/fcell.2020.608600⟩, Frontiers in cell and developmental biology, 8:608600. Frontiers Media S.A., Frontiers in Cell and Developmental Biology, Vol 8 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media SA, 2020.
-
Abstract
- Neutrophils are the first cells recruited at the site of infections, where they phagocytose the pathogens. Inside the phagosome, pathogens are killed by proteolytic enzymes that are delivered to the phagosome following granule fusion, and by reactive oxygen species (ROS) produced by the NADPH oxidase. The NADPH oxidase complex comprises membrane proteins (NOX2 and p22phox), cytoplasmic subunits (p67phox, p47phox, and p40phox) and the small GTPase Rac. These subunits assemble at the phagosomal membrane upon phagocytosis. In resting neutrophils the catalytic subunit NOX2 is mainly present at the plasma membrane and in the specific granules. We show here that NOX2 is also present in early and recycling endosomes in human neutrophils and in the neutrophil-like cell line PLB-985 expressing GFP-NOX2. In the latter cells, an increase in NOX2 at the phagosomal membrane was detected by live-imaging after phagosome closure, probably due to fusion of endosomes with the phagosome. Using super-resolution microscopy in PLB-985 WT cells, we observed that NOX2 forms discrete clusters in the plasma membrane. The number of clusters increased during frustrated phagocytosis. In PLB-985NCF1ΔGT cells that lack p47phox and do not assemble a functional NADPH oxidase, the number of clusters remained stable during phagocytosis. Our data suggest a role for p47phox and possibly ROS production in NOX2 recruitment at the phagosome.
- Subjects :
- 0301 basic medicine
Phagocyte
Endosome
[SDV]Life Sciences [q-bio]
Phagocytosis
nanoclusters
610 Medicine & health
1309 Developmental Biology
1307 Cell Biology
Cell and Developmental Biology
03 medical and health sciences
NOX2
0302 clinical medicine
NADPH oxidase complex
medicine
dSTORM
lcsh:QH301-705.5
Phagosome
NADPH oxidase
biology
Chemistry
Proteolytic enzymes
11359 Institute for Regenerative Medicine (IREM)
Cell Biology
Brief Research Report
Cell biology
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
Membrane protein
030220 oncology & carcinogenesis
biology.protein
super-resolution imaging
Developmental Biology
Subjects
Details
- ISSN :
- 2296634X
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cell and Developmental Biology
- Accession number :
- edsair.doi.dedup.....70df58c3a5e42f8c3751109f3a32b6c8
- Full Text :
- https://doi.org/10.3389/fcell.2020.608600