PRODUCTION OF HUMAN MONOCLONAL ANTIBODY TO RHESUS D ANTIGEN

Authors :: E R Huehns
L Winger
J F Harrison
M J Barlow
D H Crawford
Source :: The Lancet. 321:386-388
Publication Year :: 1983
Publisher :: Elsevier BV, 1983.
Abstract: Peripheral-blood mononuclear cells from an individual known to produce antibody to the rhesus D blood group were enriched for specific antibody-producing cells before transformation with Epstein-Barr virus. An anti-rhesus-D antibody-producing cell line (UCH D4) was obtained and cloned. Culture supernatants contained about 20 micrograms/ml of rhesus-D-specific IgG-class antibody. The antibody reactivity compared well with that of reagents used routinely for rhesus blood-grouping.