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Digital next-generation sequencing identifies low-abundance mutations in pancreatic juice samples collected from the duodenum of patients with pancreatic cancer and intraductal papillary mucinous neoplasms
- Source :
- Gut
- Publication Year :
- 2016
- Publisher :
- BMJ Publishing Group, 2016.
-
Abstract
- Objective Secretin-stimulated pancreatic juice contains DNA shed from cells lining the pancreatic ducts. Genetic analysis of this fluid may form a test to detect pancreatic ductal neoplasia. Design We employed digital next-generation sequencing (‘digital NGS’) to detect low-abundance mutations in secretin-stimulated juice samples collected from the duodenum of subjects enrolled in Cancer of the Pancreas Screening studies at Johns Hopkins Hospital. For each juice sample, digital NGS necessitated 96 NGS reactions sequencing nine genes. The study population included 115 subjects (53 discovery, 62 validation) (1) with pancreatic ductal adenocarcinoma (PDAC), (2) intraductal papillary mucinous neoplasm (IPMN), (3) controls with non-suspicious pancreata. Results Cases with PDAC and IPMN were more likely to have mutant DNA detected in pancreatic juice than controls (both p
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
endocrine system diseases
Gastroenterology
Sensitivity and Specificity
ENDOSCOPIC ULTRASONOGRAPHY
03 medical and health sciences
0302 clinical medicine
Pancreatic Juice
Predictive Value of Tests
Internal medicine
Pancreatic cancer
Medicine
Humans
Genetic Testing
Pancreas
Aged
Smad4 Protein
Intraductal papillary mucinous neoplasm
business.industry
PANCREATIC PATHOLOGY
MUTATIONS
Cancer
Sequence Analysis, DNA
Middle Aged
medicine.disease
Adenocarcinoma, Mucinous
PANCREATIC CANCER
Carcinoma, Papillary
3. Good health
Pancreatic Neoplasms
medicine.anatomical_structure
Dysplasia
030220 oncology & carcinogenesis
Pancreatic juice
Mutation
Duodenum
Population study
030211 gastroenterology & hepatology
Female
Tumor Suppressor Protein p53
business
Carcinoma, Pancreatic Ductal
Subjects
Details
- Language :
- English
- ISSN :
- 14683288 and 00175749
- Volume :
- 66
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Gut
- Accession number :
- edsair.doi.dedup.....709c92a8c2862f2a73ec603520a4962d