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Novel mechanism of gene transfection by low-energy shock wave
- Source :
- Scientific Reports, SCIENTIFIC REPORTS(5)
- Publication Year :
- 2015
- Publisher :
- Nature Publishing Group, 2015.
-
Abstract
- Extracorporeal shock wave (SW) therapy has been studied in the transfection of naked nucleic acids into various cell lines through the process of sonoporation, a process that affects the permeation of cell membranes, which can be an effect of cavitation. In this study, siRNAs were efficiently transfected into primary cultured cells and mouse tumor tissue via SW treatment. Furthermore SW-induced siRNA transfection was not mediated by SW-induced sonoporation, but by microparticles (MPs) secreted from the cells. Interestingly, the transfection effect of the siRNAs was transferable through the secreted MPs from human umbilical vein endothelial cell (HUVEC) culture medium after treatment with SW, into HUVECs in another culture plate without SW treatment. In this study, we suggest for the first time a mechanism of gene transfection induced by low-energy SW through secreted MPs and show that it is an efficient physical gene transfection method in vitro and represents a safe therapeutic strategy for site-specific gene delivery in vivo.
- Subjects :
- Male
Vascular Endothelial Growth Factor A
Small interfering RNA
animal structures
viruses
Cell
Primary Cell Culture
Mice, Nude
Neovascularization, Physiologic
Gene delivery
Biology
Transfection
Article
High-Energy Shock Waves
Cell-Derived Microparticles
Cell Line, Tumor
medicine
Human Umbilical Vein Endothelial Cells
Animals
Humans
RNA, Small Interfering
Aorta
Multidisciplinary
Neovascularization, Pathologic
fungi
Molecular biology
Mice, Inbred C57BL
Vascular endothelial growth factor A
medicine.anatomical_structure
Cell culture
Gene Knockdown Techniques
embryonic structures
Human umbilical vein endothelial cell
RNA Interference
Sonoporation
Neoplasm Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....7095c6e5fab8d36711e3cdebbe4bf142
- Full Text :
- https://doi.org/10.1038/srep12843