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The Charge Distribution in the Cytoplasmic Loop of Subunit C of the F1F0 ATPase Is a Determinant for YidC Targeting
- Source :
- The Journal of Biological Chemistry, 283(15), 9871-9877. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- YidC is a member of the Oxa1 family of proteins that facilitates the membrane insertion of a subset of inner membrane proteins in Escherichia coli. YidC acts as an insertase for membrane insertion of subunit c of the F1F0 ATP synthase (F(0)c), but the requirements for substrate recognition have remained unclear. Here, we have analyzed the role of the charged aminoacyl residues in F(0)c in YidC targeting and membrane insertion. Binding experiments demonstrate that F(0)c is targeted directly to YidC without the presence of a stable lipid surface-bound intermediate. Positive charges in the cytoplasmic loop of F(0)c are important determinants for YidC binding and subsequent membrane insertion. These data support a model in which F(0)c binds directly to YidC prior to its membrane insertion.
- Subjects :
- Models, Molecular
Protein subunit
BIOGENESIS
SRP
medicine.disease_cause
INNER MEMBRANE
Biochemistry
Protein Structure, Secondary
Substrate Specificity
PATHWAY
Protein structure
Escherichia coli
medicine
SYNTHASE
Inner membrane
Molecular Biology
MEMBRANE-PROTEIN INSERTION
ATP synthase
biology
F-0 COMPLEX
Escherichia coli Proteins
ESCHERICHIA-COLI YIDC
Cell Membrane
Membrane Transport Proteins
Cell Biology
Protein Structure, Tertiary
Loop (topology)
Amino Acid Substitution
Bacterial Proton-Translocating ATPases
Cytoplasm
biology.protein
Biophysics
BACTERIOPHAGE-M13
M13 PROCOAT
Biogenesis
Protein Binding
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 283
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....708f8b4fb29c6bc129ac9da62c9172da