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Nonribosomal peptide synthetase with a unique iterative-alternative-optional mechanism catalyzes amonabactin synthesis in Aeromonas
- Source :
- Applied Microbiology and Biotechnology, Applied Microbiology and Biotechnology, Springer Verlag, 2016, 100 (19), pp.8453-8463. ⟨10.1007/s00253-016-7773-4⟩, Applied Microbiology and Biotechnology, 2016, 100 (19), pp.8453-8463. ⟨10.1007/s00253-016-7773-4⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- Based on the exploration of data generated by genome sequencing, a bioinformatics approach has been chosen to identify the biosynthetic pathway of the siderophores produced by Aeromonas species. The amonabactins, considered as a virulence factor, represent a family of four variants of catechol peptidic siderophores containing Dhb, Lys, Gly, and an aromatic residue either Trp or Phe in a D-configuration. The synthesis operon is constituted of seven genes named amoCEBFAGH and is iron-regulated. The cluster includes genes encoding proteins involved in the synthesis and incorporation of the Dhb monomer, and genes encoding specific nonribosomal peptide synthetases, which are responsible for the building of the peptidic moiety. The amonabactin assembly line displays a still so far not described atypical mode of synthesis that is iterative, alternative, and optional. A disruption mutant in the adenylation domain of AmoG was unable to synthesize any amonabactin and to grow in iron stress conditions while a deletion of amoH resulted in the production of only two over the four forms. The amo cluster is widespread among most of the Aeromonas species, only few species produces the enterobactin siderophore.
- Subjects :
- 0301 basic medicine
Siderophore
Operon
[SDV]Life Sciences [q-bio]
030106 microbiology
Mutant
Nonribosomal peptide synthetase
Siderophores
Biology
Applied Microbiology and Biotechnology
Gene Knockout Techniques
03 medical and health sciences
chemistry.chemical_compound
Residue (chemistry)
Enterobactin
Nonribosomal peptide
Peptide Synthases
Gene
Adenylylation
chemistry.chemical_classification
NRPS
General Medicine
030104 developmental biology
chemistry
Biochemistry
Multigene Family
Amonabactin
Aeromonas
Oligopeptides
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 01757598 and 14320614
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology, Applied Microbiology and Biotechnology, Springer Verlag, 2016, 100 (19), pp.8453-8463. ⟨10.1007/s00253-016-7773-4⟩, Applied Microbiology and Biotechnology, 2016, 100 (19), pp.8453-8463. ⟨10.1007/s00253-016-7773-4⟩
- Accession number :
- edsair.doi.dedup.....708f6f1bb1926ef6bf0f89eb509faffa