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Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures
- Source :
- Journal of Visualized Experiments.
- Publication Year :
- 2016
- Publisher :
- MyJove Corporation, 2016.
-
Abstract
- Medical imaging using animal models for human diseases has been utilized for decades; however, until recently, medical imaging of diseases induced by high-consequence pathogens has not been possible. In 2014, the National Institutes of Health, National Institute of Allergy and Infectious Diseases, Integrated Research Facility at Fort Detrick opened an Animal Biosafety Level 4 (ABSL-4) facility to assess the clinical course and pathology of infectious diseases in experimentally infected animals. Multiple imaging modalities including computed tomography (CT), magnetic resonance imaging, positron emission tomography, and single photon emission computed tomography are available to researchers for these evaluations. The focus of this article is to describe the workflow for safely obtaining a CT image of a live guinea pig in an ABSL-4 facility. These procedures include animal handling, anesthesia, and preparing and monitoring the animal until recovery from sedation. We will also discuss preparing the imaging equipment, performing quality checks, communication methods from "hot side" (containing pathogens) to "cold side," and moving the animal from the holding room to the imaging suite.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Pathology
General Chemical Engineering
Operating procedures
Guinea Pigs
Computed tomography
Single-photon emission computed tomography
Animal Welfare
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Animal welfare
medicine
Medical imaging
Animals
Humans
Anesthesia
Medical physics
Personal protective equipment
General Immunology and Microbiology
medicine.diagnostic_test
business.industry
General Neuroscience
Magnetic resonance imaging
Containment of Biohazards
Magnetic Resonance Imaging
Disease Models, Animal
030104 developmental biology
Positron emission tomography
Positron-Emission Tomography
Safety
Infection
Laboratories
Tomography, X-Ray Computed
business
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....708c7f557a156dcb5c0fb3c9184ebd32