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Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth
- Source :
- Odontology. 108:532-544
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Follicular dendritic cell-secreted protein (FDC-SP) is secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium (JE). Its expression could be controlled during inflammatory process of gingiva; however, responsible mechanism for gingival overgrowth and involvement of FDC-SP in clinical condition is still unclear. We hypothesized that JE-specific genes are associated with the initiation of drug-induced gingival enlargement (DIGE) called gingival overgrowth, and investigated the changes of JE-specific gene's expression and their localization in overgrown gingiva from the patients. Immunohistochemical analysis revealed that the FDC-SP localization was spread in overgrown gingival tissues. FDC-SP mRNA levels in GE1 and Ca9-22 cells were increased by time-dependent nifedipine treatments, similar to other JE-specific genes, such as Amelotin (Amtn) and Lamininβ3 subunit (Lamβ3), whereas type 4 collagen (Col4) mRNA levels were decreased. Immunocytochemical analysis showed that FDC-SP, AMTN, and Lamβ3 protein levels were increased in GE1 and Ca9-22 cells. Transient transfection analyses were performed using luciferase constructs including various lengths of human FDC-SP gene promoter, nifedipine increased luciferase activities of -345 and -948FDC-SP constructs. These results raise the possibility that the nifedipine-induced FDC-SP may be related to the mechanism responsible for gingival overgrowth does not occur at edentulous jaw ridges.
- Subjects :
- Nifedipine
Follicular dendritic cells
biology
Gingival Overgrowth
Chemistry
Epithelial Attachment
Gingiva
Junctional epithelium
030206 dentistry
medicine.disease
Molecular biology
Gingival enlargement
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
FDC-SP
Gene expression
medicine
biology.protein
Humans
Immunohistochemistry
Luciferase
General Dentistry
Dendritic Cells, Follicular
Subjects
Details
- ISSN :
- 16181255 and 16181247
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Odontology
- Accession number :
- edsair.doi.dedup.....7075ef0e7b23f36f175c049d7d012aed