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Flow injection amperometric determination of procaine in pharmaceutical formulation using a screen-printed carbon electrode
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 43:315-319
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- A rapid and simple method for procaine determination was developed by flow injection analysis (FIA) using a screen-printed carbon electrode (SPCE) as amperometric detector. The present method is based on the amine/hydroxylamine oxidation from procaine monitored at 0.80 V on SPCE in sodium acetate solution pH 6.0. Using the best experimental conditions assigned as: pH 6.0, flow rate of 3.8 mL min −1 , sample volume of 100 μL and analytical path of 30 cm it is possible to construct a linear calibration curve from 9.0 × 10 −6 to 1.0 × 10 −4 mol L −1 . The relative standard deviation for 5.0 × 10 −5 mol L −1 procaine (15 repetitions using the same electrode) is 3.2% and detection limit calculated is 6.0 × 10 −6 mol L −1 . Recoveries obtained for procaine gave a mean values from 94.8 to 102.3% and an analytical frequency of 36 injections per hour was achieved. The method was successfully applied for the determination of procaine in pharmaceutical formulation without any pre-treatment, which are in good accordance with the declared values of manufacturer and an official method based on spectrophotometric analysis.
- Subjects :
- Calibration curve
Chemistry, Pharmaceutical
Clinical Biochemistry
Analytical chemistry
Pharmaceutical Science
Pharmaceutical formulation
Dosage form
Analytical Chemistry
Procaine
Drug Discovery
Electrochemistry
medicine
Anesthetics, Local
Electrodes
Spectroscopy
Detection limit
Flow injection analysis
Chromatography
Chemistry
Hydrogen-Ion Concentration
Carbon
Amperometry
Solutions
Calibration
Flow Injection Analysis
Indicators and Reagents
Oxidation-Reduction
Quantitative analysis (chemistry)
medicine.drug
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....70575ba56e88649677b4448e3b300ebd