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BK virus-associated infection in cerebrospinal fluid of neurological patients and mutation analysis of the complete VP1 gene in different patient groups
- Source :
- Journal of cellular physiology. 227(1)
- Publication Year :
- 2011
-
Abstract
- While BK virus (BKV) is frequently associated with pathological conditions in bone marrow and renal transplant recipients, BKV infection in neurological individuals has been rarely reported. As a result of a BKV, JCV, and SV40 large T antigen-specific multiplex PCR on 2,062 cerebrospinal fluid (CSF) samples from neurological patients suspicious of JCV infection, we identified 20 subjects with at least 1 CSF specimen positive for BKV large T antigen DNA. Because VP1 protein has been suggested to influence the biological/pathological properties of BKV, we tried to sequence the entire VP1 gene in the BKV-positive neurological patients and succeeded in 14 of the 20 neurological patients. To compare the VP1 sequence of the BKV neurological strains with that of non-neurotropic strains in other clinical situations, full-length VP1 DNA was sequenced in 15 renal and 6 bone marrow transplant recipients positive to BKV-viremia, and in 8 pregnant women as non-pathological controls. An increased (respectively, decreased) tendency for mutations in the BC loop (respectively, EF loop) was observed, and no mutations were detected in the CD, GH, and HI loops. Subtype I was predominant (93%) and compared to archetypal BKV (WW), amino acid substitutions were detected in 4/14 neurological patients, 10/15 renal transplant recipients, 3/6 bone marrow transplant patients, and in all the pregnant women. Each patient group had distinctive VP1 mutations, but these unique substitutions were not present in all patients of this group. However, molecular modeling simulations of the VP1 mutants predicted changes in protein surface properties which might affect the VP1–receptor interaction. J. Cell. Physiol. 227: 136–145, 2012. © 2011 Wiley Periodicals, Inc.
- Subjects :
- Adult
Male
Adolescent
Physiology
viruses
Clinical Biochemistry
DNA Mutational Analysis
Molecular Sequence Data
Biology
medicine.disease_cause
Polymerase Chain Reaction
law.invention
Young Adult
Cerebrospinal fluid
Antigen
law
Central Nervous System Diseases
Pregnancy
medicine
Humans
Amino Acid Sequence
Child
Gene
Pathological
Polymerase chain reaction
Aged
Polyomavirus Infections
virus diseases
Cell Biology
Middle Aged
BK virus
Tumor Virus Infections
medicine.anatomical_structure
BK Virus
Child, Preschool
Immunology
DNA, Viral
Mutation testing
Capsid Proteins
Female
Bone marrow
Multiplex Polymerase Chain Reaction
Subjects
Details
- ISSN :
- 10974652
- Volume :
- 227
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of cellular physiology
- Accession number :
- edsair.doi.dedup.....704acb64924bbd9fa58b40f3313e6ed0