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Isolation of Mouse and Human Tumor-Associated Macrophages
- Source :
- Advances in Experimental Medicine and Biology ISBN: 9783319266640
- Publication Year :
- 2016
-
Abstract
- The tumor microenvironment is a complex network of cells that support tumor progression and malignancy. It has been demonstrated that tumor cells can educate the immune system to promote a tumor-friendly environment. Among all these immune cells, tumor-associated macrophages (TAMs) are well represented and their presence in mouse models has been shown to promote tumor progression and metastasis. These effects are through the stimulation of angiogenesis, enhancement of tumor cell invasion and intravasation, immunosuppression, and at the metastatic site tumor cell extravasation and growth. However, the precise mechanisms are not fully understood. Furthermore there is limited information on TAMs derived from human cancers. For this reason it is important to be able to extract TAMs from tumors in order to compare their phenotypes, functions, and transcriptomes with normal resident tissue macrophages. Isolation of these cells is challenging due to the lack of markers and standardized protocols. Here we show an optimized protocol for the efficient isolation and extraction of resident macrophages and TAMs from human and mouse tissues by using multicolor flow cytometry. These protocols allow for the extraction of thousands of macrophages in less than 5 h from tissues as small as half a gram. The isolated macrophages can then be used for both “omics” and in vitro studies.
- Subjects :
- 0301 basic medicine
Angiogenesis
Cell
Breast Neoplasms
Cell Separation
Biology
Hemolysis
Article
Flow cytometry
Metastasis
03 medical and health sciences
Mice
Immune system
Neoplasms
medicine
Animals
Humans
Tumor microenvironment
medicine.diagnostic_test
Macrophages
Intravasation
medicine.disease
Flow Cytometry
030104 developmental biology
medicine.anatomical_structure
Tumor progression
Immunology
Cancer research
Female
Subjects
Details
- Language :
- English
- ISBN :
- 978-3-319-26664-0
- ISBNs :
- 9783319266640
- Database :
- OpenAIRE
- Journal :
- Advances in Experimental Medicine and Biology ISBN: 9783319266640
- Accession number :
- edsair.doi.dedup.....70375d6646041112d4026b14cc4bedfe