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Detection of EGFR mutations with mutation-specific antibodies in stage IV non-small-cell lung cancer
- Source :
- Journal of Translational Medicine, Journal of Translational Medicine, Vol 8, Iss 1, p 135 (2010)
- Publication Year :
- 2010
-
Abstract
- Background Immunohistochemistry (IHC) with mutation-specific antibodies may be an ancillary method of detecting EGFR mutations in lung cancer patients. Methods EGFR mutation status was analyzed by DNA assays, and compared with IHC results in five non-small-cell lung cancer (NSCLC) cell lines and tumor samples from 78 stage IV NSCLC patients. Results IHC correctly identified del 19 in the H1650 and PC9 cell lines, L858R in H1975, and wild-type EGFR in H460 and A549, as well as wild-type EGFR in tumor samples from 22 patients. IHC with the mAb against EGFR with del 19 was highly positive for the protein in all 17 patients with a 15-bp (ELREA) deletion in exon 19, whereas in patients with other deletions, IHC was weakly positive in 3 cases and negative in 9 cases. IHC with the mAb against the L858R mutation showed high positivity for the protein in 25/27 (93%) patients with exon 21 EGFR mutations (all with L858R) but did not identify the L861Q mutation in the remaining two patients. Conclusions IHC with mutation-specific mAbs against EGFR is a promising method for detecting EGFR mutations in NSCLC patients. However these mAbs should be validated with additional studies to clarify their possible role in routine clinical practice for screening EGFR mutations in NSCLC patients.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Lung Neoplasms
medicine.drug_class
Antibodies, Neoplasm
DNA Mutational Analysis
lcsh:Medicine
Monoclonal antibody
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
Exon
Antibody Specificity
Carcinoma, Non-Small-Cell Lung
Cell Line, Tumor
medicine
Humans
Epidermal growth factor receptor
Lung cancer
Aged
Neoplasm Staging
Sequence Deletion
Medicine(all)
Aged, 80 and over
Mutation
biology
business.industry
Biochemistry, Genetics and Molecular Biology(all)
Research
lcsh:R
Antibodies, Monoclonal
General Medicine
Exons
Middle Aged
medicine.disease
Immunohistochemistry
respiratory tract diseases
ErbB Receptors
Cell culture
Cancer research
biology.protein
Female
Antibody
business
Subjects
Details
- ISSN :
- 14795876
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of translational medicine
- Accession number :
- edsair.doi.dedup.....70153c322c71a794f2de5a6fbff5f620