Xenogeneic Transfer of Adult Quail (Coturnix coturnix) Spermatogonial Stem Cells to Embryonic Chicken (Gallus gallus) Hosts: A Model for Avian Conservation1

As advanced reproductive technologies have become routine for domesticated species, they have begun to be applied in the field of endangered species conservation. For avian conservation, the most promising technology is the transfer of germ stem cells of exotic species to domestic hosts for the production of gametes. In this study, adult quail (model for exotic species) spermatogonial stem cells were xenogeneically transferred to stages 14-17 chicken host embryos. Fluorescent cellular dyes, quail-specific antibodies, and quail-specific quantitative PCR confirmed donor cell migration to and colonization of the host gonadal ridge. Donor-derived cells were observed by fluorescent microscopy in the caudal area as early as 2 h after injection, in the gonadal ridge at 4 h after injection, as well as in the gonads of stages 35-38 host embryos. Four of eight donor-derived cell flow cytometry-positive host gonads were confirmed by quantitative PCR using quail-specific primers. There was no statistically significant effect of host stage of injection, host gonad isolation stage, or host sex on the number of hosts positive for donor cells or the percent of donor-derived cells per positive gonad. Donor-derived cells isolated from stages 35-38 host gonads costained with the germ stem cell marker SSEA-1, indicating that the donor-derived cells have maintained stem cell-ness. This is the first study to suggest that it is feasible to rescue adult germ stem cells of deceased birds to prolong the reproductive lifespan of critically endangered species or genetically valuable individuals by transferring them to an embryonic chicken host.