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Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019), Scientific Reports
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group, 2019.
-
Abstract
- This project was supported by grants from the British Heart Foundation (PG/11/71/29091 and PG/16/68/31991) and the Orthopaedic Institute Ltd., (RJAH Orthopaedic Hospital, Oswestry, UK). Nesprins, nuclear envelope spectrin-repeat proteins encoded by the SYNE1 and SYNE2 genes, are involved in localization of nuclei. The short isoform, nesprin-1-alpha2, is required for relocation of the microtubule organizer function from centromeres to the nuclear rim during myogenesis. Using specific antibodies, we now show that both nesprin-1-alpha2 and nesprin-1-giant co-localize with kinesin at the junctions of concatenated nuclei and at the outer poles of nuclear chains in human skeletal myotubes. In adult muscle, nesprin-1-alpha2 was found, together with kinesin, only on nuclei associated with neuromuscular junctions, whereas all adult cardiomyocyte nuclei expressed nesprin-1-alpha2. In a proteomics study, kinesin heavy and light chains were the only significant proteins in myotube extracts pulled down by nesprin-1-alpha2, but not by a mutant lacking the highly-conserved STAR domain (18 amino-acids, including the LEWD motif). The results support a function for nesprin-1-alpha2 in the specific localization of skeletal muscle nuclei mediated by kinesins and suggest that its primary role is at the outer nuclear membrane. Publisher PDF
- Subjects :
- Gene isoform
Proteomics
Nuclear Envelope
0299 Other Physical Sciences
Muscle Fibers, Skeletal
Neuromuscular Junction
Kinesins
lcsh:Medicine
Nerve Tissue Proteins
0601 Biochemistry and Cell Biology
Muscle Development
Neuromuscular junction
Article
03 medical and health sciences
0302 clinical medicine
Microtubule
Centromere
medicine
Animals
Humans
Protein Isoforms
Muscle, Skeletal
lcsh:Science
030304 developmental biology
Cell Nucleus
0303 health sciences
Multidisciplinary
Nesprin
Chemistry
Myogenesis
Microfilament Proteins
lcsh:R
Skeletal muscle
Gene Expression Regulation, Developmental
DAS
Neuromuscular disease
Cell biology
Cytoskeletal Proteins
medicine.anatomical_structure
Differentiation
Mutation
RC0321
Kinesin
lcsh:Q
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....700aace8ad766270eb0f2dfd3dbc91b6