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Oral Recombinant Methioninase, Combined With Oral Caffeine and Injected Cisplatinum, Overcome Cisplatinum-Resistance and Regresses Patient-derived Orthotopic Xenograft Model of Osteosarcoma
- Source :
- Anticancer Research. 39:4653-4657
- Publication Year :
- 2019
- Publisher :
- Anticancer Research USA Inc., 2019.
-
Abstract
- Background/aim Osteosarcoma is a recalcitrant neoplasm which occurs predominantly in adolescents and young adults. Recently, using a patient-derived orthotopic xenograft (PDOX) model of malignant soft-tissue sarcoma (STS), we showed that oral recombinant methioninase (o-rMETase), in combination with caffeine, was more efficacious than o-rMETase alone in inhibiting STS tumor growth. In the present report, we determined the efficacy of o-rMETase combined with oral caffeine on a cisplatinum (CDDP)-resistant osteosarcoma PDOX model. Materials and methods Osteosarcoma PDOX models were randomly divided into seven treatment groups (6 mice in each group): untreated control; CDDP alone; o-rMETase alone; o-rMETase with caffeine; CDDP plus o-rMETase; CDDP plus caffeine; and CDDP plus o-rMETase with caffeine. Tumor size and body weight were measured throughout the treatment. Results Tumors regressed after treatment with CDDP plus o-rMETase with caffeine. Tumors treated with CDDP plus o-rMETase with caffeine also had the most necrosis. Conclusion The combination of o-rMETase and caffeine together with first-line chemotherapy was efficacious for drug-resistant osteosarcoma and has clinical potential in the treatment of this highly-resistant neoplasm.
- Subjects :
- inorganic chemicals
Antimetabolites, Antineoplastic
Cancer Research
Necrosis
medicine.medical_treatment
Administration, Oral
law.invention
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
law
Caffeine
Animals
Humans
Medicine
Neoplasm
neoplasms
Osteosarcoma
Chemotherapy
Tumor size
business.industry
General Medicine
medicine.disease
Xenograft Model Antitumor Assays
female genital diseases and pregnancy complications
Tumor Burden
Carbon-Sulfur Lyases
Disease Models, Animal
Oncology
chemistry
Drug Resistance, Neoplasm
030220 oncology & carcinogenesis
Recombinant DNA
Cancer research
Sarcoma
Cisplatin
medicine.symptom
business
Subjects
Details
- ISSN :
- 17917530 and 02507005
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Anticancer Research
- Accession number :
- edsair.doi.dedup.....6ffd113251c30c1a50ed37b6e4093e87